Kai Johnsson

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Improving split-HaloTag through computational protein engineering

Split-HaloTag can be used to transform transient molecular interactions into permanent marks through chemical labeling, thereby enabling the recording of transient physiological events in individual cells. However, applications of split-HaloTag-based recor ...

WILEY2025

Exploiting Covalent Chemical Labeling with Self-Labeling Proteins

Kai Johnsson

The visualization and manipulation of proteins in live cells are critical for studying complex biological processes. Self-labeling proteins do so by enabling the specific and covalent attachment of synthetic probes, offering unprecedented flexibility in th ...

Annual Reviews2025

Molecular recording of cellular protein kinase activity with chemical labeling

Kai Johnsson

Protein kinases control most cellular processes and aberrant kinase activity is involved in numerous diseases. Here we introduce molecular recorders of kinase activities for later analysis to investigate the link between specific kinase activities and cell ...

2025

A Silicon Rhodamine-fused Glibenclamide to Label and Detect Malaria-infected Red Blood Cells

Kai Johnsson, Luc Reymond, Johannes Broichhagen

The malaria parasite Plasmodium falciparum affects the lives of millions of people worldwide every year. The detection of replicating parasites within human red blood cells is of paramount importance, requiring appropriate diagnostic tools. Herein, we desi ...

WILEY-V C H VERLAG GMBH2025

SNAP-tag2 for faster and brighter protein labeling

Kai Johnsson

SNAP-tag is a powerful tool for labeling proteins with synthetic fluorophores in bioimaging. However, its utility in live-cell applications can be constrained by its relatively slow labeling kinetics and the limited cell permeability of its substrates. Her ...

2025

Recording physiological history of cells with chemical labeling

Kai Johnsson, Julien Hiblot, Ling Hai

Recordings of the physiological history of cells provide insights into biological processes, yet obtaining such recordings is a challenge. To address this, we introduce a method to record transient cellular events for later analysis. We designed proteins t ...

American Association for the Advancement of Science2024

AspSnFR: A genetically encoded biosensor for real-time monitoring of aspartate in live cells

Kai Johnsson

Aspartate is crucial for nucleotide synthesis, ammonia detoxification, and maintaining redox balance via the malate-aspartate-shuttle (MAS). To disentangle these multiple roles of aspartate metabolism, tools are required that measure aspartate concentratio ...

CELL PRESS2024

Metal-free introduction of primary sulfonamide into electron-rich aromatics

Kai Johnsson

We report herein a direct and practical synthesis of arylsulfonamides from electron-rich aromatic compounds by using in situ generated N-sulfonylamine as the active electrophile. Substrates include derivatives of aniline, indole, pyrrole, furan, styrene an ...

ROYAL SOC CHEMISTRY2024

Fluorogenic Rhodamine-Based Chemigenetic Biosensor for Monitoring Cellular NADPH Dynamics

Kai Johnsson

Ratiometric biosensors employing Forster Resonance Energy Transfer (FRET) enable the real-time tracking of metabolite dynamics. Here, we introduce an approach for generating a FRET-based biosensor in which changes in apparent FRET efficiency rely on the an ...

AMER CHEMICAL SOC2024

Low temperature and mTOR inhibition favor stem cell maintenance in human keratinocyte cultures

Kai Johnsson, Yann Barrandon, Johannes Alexander Mosig, Thomas Michael Braschler, Ariane Rochat, Jean-Baptiste Bureau, Fahd Azzabi Zouraq, Mako Kamiya

Adult autologous human epidermal stem cells can be extensively expanded ex vivo for cell and gene therapy. Identifying the mechanisms involved in stem cell maintenance and defining culture conditions to maintain stemness is critical, because an inadequate ...

WILEY2023

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