Two-photon excitation microscopyTwo-photon excitation microscopy (TPEF or 2PEF) is a fluorescence imaging technique that is particularly well-suited to image scattering living tissue of up to about one millimeter in thickness. Unlike traditional fluorescence microscopy, where the excitation wavelength is shorter than the emission wavelength, two-photon excitation requires simultaneous excitation by two photons with longer wavelength than the emitted light. The laser is focused onto a specific location in the tissue and scanned across the sample to sequentially produce the image.
Apex graphIn graph theory, a branch of mathematics, an apex graph is a graph that can be made planar by the removal of a single vertex. The deleted vertex is called an apex of the graph. It is an apex, not the apex because an apex graph may have more than one apex; for example, in the minimal nonplanar graphs K_5 or K_3,3, every vertex is an apex. The apex graphs include graphs that are themselves planar, in which case again every vertex is an apex. The null graph is also counted as an apex graph even though it has no vertex to remove.
