Accelerated autofocusing of off-axis holograms using critical sampling

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Substrates for Improved Live-Cell Fluorescence Labeling of SNAP-tag

Kai Johnsson, Luc Reymond, Grazvydas Lukinavicius

The SNAP-tag labeling technology provides a simple, robust, and versatile approach to the imaging of fusion proteins for a wide range of experimental applications. Owing to the specific and covalent nature of the labeling reaction, SNAP-tag is well suited ...

Bentham Science Publ Ltd2013

Contrast Enhancement in Optical Coherence Microscopy

Christophe Pache

Optical Coherence Microscopy (OCM) is a three-dimensional imaging technique that provides cross-sectional views of the subsurface microstructure of biological tissue, with a high axial and lateral resolution. In OCM, a low time coherence light source is sp ...

EPFL2012

Holographic Second Harmonic Generation Microscopy

Etienne Shaffer

This dissertation is dedicated to the application of digital holography methods to second harmonic generation microscopy, and to the development of the resulting imaging technique: holographic second harmonic generation microscopy. Second harmonic generati ...

EPFL2011

Fast Single-Photon Avalanche Diode Arrays for Laser Raman Spectroscopy

Edoardo Charbon

We incorporate newly developed solid-state detector technology into time-resolved laser Raman spectroscopy, demonstrating the ability to distinguish spectra from Raman and fluorescence processes. As a proof of concept, we show fluorescence rejection on hig ...

Optical Society of America2011

Study of the Optical UV-Visible Electron Induced Luminescence of Small Size Selected Metal Clusters in Rare Gas Solids

Alexandre Rydlo

The optical properties of small size selected metal clusters have been studied by fluorescence spectroscopy. Cun (n = 1-5, 8), Agn (n = 1-5, 8, 9, 20), Aun (n = 1-5, 8) and Nin (n = 1-3) clusters embedded in condensed neon are photoionized by VUV photons i ...

EPFL2010

Microscopy image resolution improvement by deconvolution of complex fields

Christian Depeursinge, Nicolas Pavillon, Muhammed Fatih Toy, Yann Jérôme Michel Pascal Cotte

Based on truncated inverse filtering, a theory for deconvolution of complex fields is studied. The validity of the theory is verified by comparing with experimental data from digital holographic microscopy (DHM) using a high-NA system (NA=0.95). Comparison ...

Optical Society of America2010

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