Lecture

Immunoprecipitation: Isolating Proteins of Interest

Description

This lecture covers the process of immunoprecipitation, which involves using specific antibodies to isolate a target protein from a cell lysate. The antibodies are bound to solid supports like magnetic or polystyrene beads, allowing for the separation and purification of the protein of interest. By changing the buffer conditions, the protein can be eluted and purified for further use, a technique applicable to chromatin and esters.

In MOOCs (6)

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Official source

https://mediaspace.epfl.ch/media/0_0yd8lbir

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