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This lecture focuses on the analysis of carbonates and bicarbonates in soil using a Berna calcimeter system, which involves adding acid to the soil sample to release CO2, measuring the volume of CO2 released, and calculating the initial quantity of carbonates and bicarbonates. The process includes weighing the soil sample, adding acid, capturing the released CO2 in a measurement tube, and adjusting for temperature and atmospheric pressure to apply the ideal gas law.