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This lecture covers the process of DNA replication in eukaryotic cells, focusing on the formation of pre-replicative complexes at replication origins, the activation and inactivation of key proteins such as Cdk and APC/C, and the mechanisms that limit DNA replication to once per cell cycle. The lecture also discusses the role of licensing factors, the assembly of replication initiation complexes, and the regulation of replication through phosphorylation and proteolysis.