Transgenic Mice: Cre-Lines and Optogenetics

This lecture covers the use of transgenic mice with Cre-lines to label, record, or manipulate specific neuronal cell types. It also explores optogenetics, a technique using microbial opsins like Channelrhodopsin-2 and Halorhodopsin to control neural activity. The lecture delves into transcriptomics, proteomics, and single-cell RNA sequencing techniques in neuroscience research, emphasizing the importance of statistics and machine learning in analyzing large datasets. Additionally, it discusses the Allen Brain Atlas and the BigBrain project, showcasing high-resolution 3D models of the human brain. The lecture concludes with insights into electrode technologies, synaptic mapping, and the integration of different scales in brain research.