Biosensors: Electrode Technology

This lecture covers the technology behind biosensors, focusing on electrode design and fabrication. Topics include the use of Ag/AgCl reference electrodes, planar electrode preparation, potentiometry, linear and cyclic voltammetry, and conductometry. The instructor explains the construction of various electrodes, such as the Ag/AgCl reference electrode and the Pt electrode, and their applications in biological fluids. Additionally, the lecture delves into the principles of amperometry, adsorption current, and the Clark cell for measuring dissolved oxygen concentrations. Various immobilization techniques for enzymes in biosensors are also discussed, including cross-linking, gel entrapment, and fiber entrapment.