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This lecture covers the processes of methanogenesis, acetogenesis, and methane oxidation, focusing on the production of biogas from organic waste in anaerobic digesters. It explains the different types of methanogens, their habitats, and the enzymes involved in methanogenesis. The lecture also delves into the co-enzymes and electron transfer mechanisms essential for these processes, such as Methanopterin, Coenzyme M, and Coenzyme F430. Additionally, it explores the pathways for hydrogenotrophic, methanol, and aceticlastic methanogenesis, detailing the reactions and energy generation involved. The lecture concludes with a discussion on specific methanogenic species like Methanocaldococcus and Methanosarcina, highlighting their unique characteristics and habitats.