Proteomics in Neuroscience: Techniques and Applications

This lecture introduces the field of proteomics within the context of neuroscience, emphasizing the importance of proteins in cellular functions. The instructor discusses the central dogma of molecular biology, highlighting the flow of information from DNA to RNA to proteins. The lecture covers various omics technologies, particularly focusing on mass spectrometry and its applications in analyzing proteomes, modifications, and interactions. The significance of translatomics is also addressed, detailing how translation is regulated and the interaction between mRNA and proteins. The scope of neuroproteomics is explored, including the study of proteomes at different levels, from tissues to organelles, and its relevance to neurodegenerative diseases. The challenges faced in proteomics, such as complexity and dynamic range, are discussed, emphasizing the need for advanced techniques to detect and analyze proteins effectively. Overall, the lecture provides a comprehensive overview of how proteomics contributes to understanding cellular processes and the implications for neuroscience research.