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This lecture covers the principles of relaxation and structure determination in NMR spectroscopy. Topics include nuclear spin relaxation induced by fluctuating magnetic fields, anisotropic NMR interactions, NOESY experiments for distance measurements, and the protocol for protein structure determination. The instructor explains the Redfield theory, dipolar interactions, and the Abragam formulation. The lecture also delves into steady-state and transient NOE experiments, molecular dynamics, and the role of relaxation times in determining molecular structure and dynamics.