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This lecture covers bottom-up methods in nanoscience, focusing on instrumentation like AFM and STM, destructive exposure, manipulation with SIM, nanostructures, conductivity of a molecular wire, STM-induced chemical reactions, molecular beam epitaxy, thin film growth, self-organized growth at surfaces, surface tensions, Stranski-Krastanov growth of semiconductor QDs, hydrogen bonding, solution-based nanocrystal synthesis, possible applications of copper nanocrystals, nanostructure fabrication, self-assembly, supramolecular chemistry, hydrogen-bonded networks, molecular design, and self-assembly of S-layers. The lecture explores the control of structures through temperature, symmetry, and surface chemistry, as well as the self-assembly of S-layers at the solid-liquid interface.