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This lecture covers the fundamental concepts of tissue optics, including tissue optical parameters, refractive index, absorption, and scattering mechanisms. The instructor discusses the interaction of light with biological tissues, the role of chromophores, and the impact of scattering on light propagation. Various biological chromophores and their molar extinction coefficients are explored, along with the limitations of Lambert-Beer's law. The lecture delves into the mechanisms of Rayleigh and Mie scattering, as well as the significance of scattering anisotropy. Additionally, the importance of scattering phase functions, such as the Heyney Greenstein approximation, is highlighted.