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This lecture covers the fundamentals of signals, instruments, and systems, starting with the Analog-Digital Converter (ADC) process, Fourier series, and Fourier Transform. It delves into sampling in both time and frequency domains, discussing the Nyquist-Shannon Theorem, signal reconstruction techniques, and the concept of aliasing. The instructor explains the importance of band-limited interpolation, signal reconstruction in practice, and the implications of sampling frequency on signal quality. Practical examples and illustrations are provided to demonstrate the effects of aliasing and the necessity of anti-alias filters.