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Publication
Are you an EPFL student looking for a semester project?
Work with us on data science and visualisation projects, and deploy your project as an app on top of Graph Search.
In this work we present the first results on a 'label-free' DNA analysis system based on hydrogenated amorphous silicon (a-Si:H) thin film sensors. The detection mechanism is based on absorbance measurements performed on DNA molecules in a buffer solution. The experiments made with single-stranded 30-mer DNA at different concentrations showed an excellent linearity over three orders of magnitude. The extrapolated minimum quantity (expressed as concentration - optical path product) detectable by our set-up was 2 nmol×cm, limited by short term variations of the UV source intensity. Furthermore, relying on the hypo-chromic effect it is possible to distinguish between single- and double-stranded DNA chains. This was observed performing DNA hybridization experiment on a functionalized glass substrate.
Sandrine Gerber, Mounir Driss Mensi, Perrine Agnes Edith Robin, Alireza Kavand, Lucas Mayoraz
Sebastian Maerkl, Nicolas Rémi Adam, Jonathan Selz