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Publication
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Work with us on data science and visualisation projects, and deploy your project as an app on top of Graph Search.
The fluorescence decay of high-affinity nonratiometric Ca2+ indicator Oregon Green BAPTA-1 (OGB-1) is analyzed with unprecedented temporal resolution in the two-photon excitation regime. A triple exponential decay is shown to best fit the fluorescence dynamics of OGB-1. We provide a model for accurate measurements of the free Ca2+ concentration and dissociation constants of nonratiometric calcium indicators.
Arianna Marchioro, Marie Bischoff
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