Concept
Gene targeting
Related publications (101)
Human shelterin protein POT1 prevents severe telomere instability induced by homology-directed DNA repair
Joachim Lingner, Anna-Sophia Briod
The evolutionarily conserved POT1 protein binds single-stranded G-rich telomeric DNA and has been implicated in contributing to telomeric DNA maintenance and the suppression of DNA damage checkpoint signaling. Here, we explore human POT1 function through g ...
WILEY2020Transcription Factors and DNA Play Hide and Seek
Transcription factors (TFs) bind to specific DNA motifs to regulate the expression of target genes. To reach their binding sites, TFs diffuse in 3D and perform local motions such as 1D sliding, hopping, or intersegmental transfer. TF-DNA interactions depen ...
2020Assessing Public Opinion on CRISPR-Cas9: Combining Crowdsourcing and Deep Learning
Background: The discovery of the CRISPR-Cas9-based gene editing method has opened unprecedented new potential for biological and medical engineering, sparking a growing public debate on both the potential and dangers of CRISPR applications. Given the speed ...
JMIR PUBLICATIONS, INC2020A complex regulatory landscape involved in the development of mammalian external genitals
Denis Duboule, Lucille Delisle, Christopher Chase Bolt
Developmental genes are often controlled by large regulatory landscapes matching topologically associating domains (TADs). In various contexts, the associated chromatin backbone is modified by specific enhancer-enhancer and enhancer-promoter interactions. ...
2020DPPA2 and DPPA4 are necessary to establish a 2C‐like state in mouse embryonic stem cells
Didier Trono, Julien Léonard Duc, Alexandre Coudray, Alberto De Iaco
After fertilization of the transcriptionally silent oocyte, expression from both parental chromosomes is launched through zygotic genome activation (ZGA), occurring in the mouse at the 2-cell (2C) stage. Among the first elements to be transcribed are the D ...
2019Tumor-targeted 4-1BB agonists for combination with T cell bispecific antibodies as off-the-shelf therapy
Henning Paul-Julius Stahlberg, Thomas Hofer, Wei Xu
Endogenous costimulatory molecules on T cells such as 4-1BB (CD137) can be leveraged for cancer immunotherapy. Systemic administration of agonistic anti-4-1BB antibodies, although effective preclinically, has not advanced to phase 3 trials because they hav ...
American Association for the Advancement of Science (AAAS)2019Impact of genome architecture on the functional activation and repression of Hox regulatory landscapes
Denis Duboule, Lucille Delisle, Edgardo Rodriguez Carballo, Nayuta Yakushiji
Background The spatial organization of the mammalian genome relies upon the formation of chromatin domains of various scales. At the level of gene regulation in cis, collections of enhancer sequences define large regulatory landscapes that usually match wi ...
2019Gene Editing Preserves Visual Functions in a Mouse Model of Retinal Degeneration
Diego Ghezzi, Paola Vagni, Naïg Aurelia Ludmilla Chenais, Tommaso Marchetti
Inherited retinal dystrophies (IRDs) are a large and heterogeneous group of degenerative diseases caused by mutations in various genes. Given the favorable anatomical and immunological characteristics of the eye, gene therapy holds great potential for thei ...
2019A new approach to Cas9-based genome editing in Aspergillus niger that is precise, efficient and selectable
Aspergillus niger and other filamentous fungi are widely used in industry, but efficient genetic engineering of these hosts remains nascent. For example, while molecular genetic tools have been developed, including CRISPR/Cas9, facile genome engineering of ...
2019Gene drives for schistosomiasis transmission control
Schistosomiasis is one of the most important and widespread neglected tropical diseases (NTD), with over 200 million people infected in more than 70 countries; the disease has nearly 800 million people at risk in endemic areas. Although mass drug administr ...
2019