Concept
Protein splicing
Related publications (38)
Development of tools and methods for protein identification: From single molecules to in vivo applications.
As the fundamental machinery orchestrating cellular functions, proteins influence the state of every cell profoundly. As cells exhibit significant variations from one to another, analyzing the proteome on a single-cell level is imperative to unravel their ...
EPFL2024Synthesis and application of bifunctional hypervalent iodine reagents for peptide modification.
Protein-protein interactions (PPIs) are responsible for regulating many biological processes in human bodies. PPIs often have a large binding site and often interact through an alpha helical segment. Therefore, the inhibition of PPIs can be difficult using ...
EPFL2023A minimalistic cyclic ice-binding peptide from phage display
Harm-Anton Klok, Luciano Andres Abriata, Corey Alfred Stevens, Matthew Gibson, Xudong Kong
Developing molecules that emulate the properties of naturally occurring ice-binding proteins (IBPs) is a daunting challenge. Rather than relying on the (limited) existing structure–property relationships that have been established for IBPs, here we report ...
2021The box C/D snoRNP assembly factor Bcd1 interacts with the histone chaperone Rtt106 and controls its transcription dependent activity
Biogenesis of eukaryotic box C/D small nucleolar ribonucleoproteins initiates co-transcriptionally and requires the action of the assembly machinery including the Hsp90/R2TP complex, the Rsa1p:Hit1p heterodimer and the Bcd1 protein. We present genetic inte ...
NATURE RESEARCH2021The functional proteome landscape of Escherichia coli
Camille Véronique Bernadette Goemans
Recent developments in high-throughput reverse genetics1,2 have revolutionized our ability to map gene function and interactions3,4,5,6. The power of these approaches depends on their ability to identify functionally associated genes, which elicit similar ...
2020Protein Engineering Tools to Explore the Function of Protein Post-Translational Modifications for Chromatin and Microtubule Cytoskeletal Biology
Protein post-translational modifications (PTMs) play a crucial role in expanding the protein diversity and are one of the major mechanisms through which cells respond to ever changing environmental cues. The function of two of the most important cellular c ...
EPFL2019A bi-terminal protein ligation strategy to probe chromatin structure during DNA damage
Beat Fierz, Sinan Kilic, Iuliia Boichenko, Carolin Christine Lechner
The cellular response to DNA damage results in a signaling cascade that primes chromatin for repair. Combinatorial post-translational modifications (PTMs) play a key role in this process by altering the physical properties of chromatin and recruiting downs ...
2018Development of microfluidic tools and methods for the biochemical characterization of scFv and Cas9 affinity reagents and their applications in molecular detection
Recent progress in protein engineering, empowered by the rapidly evolving fields of synthetic biology and DNA synthesis, has enabled the generation of various custom-designed proteins. Their thorough analysis has led to applications in areas including medi ...
EPFL2018Evolution of an intricate J-protein network driving protein disaggregation in eukaryotes
Paolo De Los Rios, Alessandro Barducci, Duccio Malinverni
Hsp70 participates in a broad spectrum of protein folding processes extending from nascent chain folding to protein disaggregation. This versatility in function is achieved through a diverse family of J-protein cochaperones that select substrates for Hsp70 ...
Elife Sciences Publications Ltd2017Heterochromatin protein 1 recruitment and chromatin conformational dynamics at the single-molecule level
Chromatin is the template on which DNA-associated transactions take place in eukaryotic organisms. Nucleosomes consisting of the four histones H2A, H2B, H3 and H4 each organize ~150bp of DNA and constitute a first layer of chromatin. The three-dimensional ...
EPFL2017