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Several methods are available to manipulate bacterial chromosomes(1-3). Most of these protocols rely on the insertion of conditionally replicative plasmids (e.g. harboring pir-dependent or temperature-sensitive replicons(1,2)). These plasmids are integrate ...
Bicyclic peptides binding to targets of interest can be isolated from combinatorial libraries using a phage display-based approach. In a first project of this thesis, we aimed at generating bicyclic peptide inhibitors of matrix metalloproteinases (MMPs), a ...
Background: Zinc Finger Nucleases (ZFNs) are man-made restriction enzymes useful for manipulating genomes by cleaving target DNA sequences. ZFNs allow therapeutic gene correction or creation of genetically modified model organisms. ZFN specificity is not a ...
Densely arrayed skeletal myotubes are activated individually and as a group using precise optical stimulation with high spatiotemporal resolution. Skeletal muscle myoblasts are genetically encoded to express a light-activated cation channel, Channelrhodops ...
Three-dimensional finite element simulations are used to investigate the role of serrated flow on the strain at the onset of necking in a cylindrical uniaxial tension specimen. The material is idealized using a modified form of the McCormick constitutive e ...
Performance on a target can be strongly modified by context. For example, vernier offset discrimination is strongly deteriorated by neighboring flankers. Performance is worst when the flankers have the same length as the vernier. Surprisingly, performance ...
Polymer brushes grafted by surface-initiated atom transfer radical polymerization (SI-ATRP) from the surface of Ir-based microelectrode arrays are explored as a platform for the fabrication of sensory coatings for the voltammetric detection of Hg2+. The po ...
In open-channel bends, interactions between streamwise flow, curvature-induced secondary flow and sediment transport lead to a typical bar-pool bed topography. Erosion occurs near the outer bank that can endanger structures, whereas deposition occurs near ...
This technical report provides supplementary information for the optimization problems defined for Stable Estimator of Dynamical Systems (SEDS). Reading of this report is not necessary for researchers who only want to use SEDS learning algorithm. The repor ...
Mammalian cells in bioreactors as production host are the focus of this review. We wish to briefly describe today's technical status and to highlight emerging trends in the manufacture of recombinant therapeutic proteins, focusing on Chinese hamster ovary ...