Publications related to Acid-fastness

A general strategy to develop fluorogenic polymethine dyes for bioimaging

Fluorescence imaging is an invaluable tool to study biological processes and further progress depends on the development of advanced fluorogenic probes that reach intracellular targets and label them with high specificity. Excellent fluorogenic rhodamine d ...

Berlin2023

Development and validation of a multiplex real-time qPCR assay using GMP-grade reagents for leprosy diagnosis

Stewart Cole, Charlotte Avanzi, Thyago Leal Calvo

Author summaryLeprosy is a chronic dermato-neurological disease caused by Mycobacterium leprae, an obligate intracellular bacterium. Diagnosis of leprosy often relies on skin examinations for clinical signs, bacilli staining from skin smears and invasive s ...

PUBLIC LIBRARY SCIENCE2022

FasR Regulates Fatty Acid Biosynthesis and Is Essential for Virulence of Mycobacterium tuberculosis

Stewart Cole, Claudia Sala

Mycobacterium tuberculosis, the etiologic agent of human tuberculosis, is the world's leading cause of death from an infectious disease. One of the main features of this pathogen is the complex and dynamic lipid composition of the cell envelope, which adap ...

FRONTIERS MEDIA SA2020

Infrared nanospectroscopic mapping of a single metaphase chromosome

Giovanni Dietler, Andrzej Kulik, Francesco Simone Ruggeri, Ewelina Wioletta Lipiec

The integrity of the chromatin structure is essential to every process occurring within eukaryotic nuclei. However, there are no reliable tools to decipher the molecular composition of metaphase chromosomes. Here, we have applied infrared nanospectroscopy ...

Oxford University Press (OUP)2019

Increased drug permeability of a stiffened mycobacterial outer membrane in cells lacking MFS transporter Rv1410 and lipoprotein LprG

Georg Fantner, Haig Alexander Eskandarian

The major facilitator superfamily transporter Rv1410 and the lipoprotein LprG (Rv1411) are encoded by a conserved two-gene operon and contribute to virulence in Mycobacterium tuberculosis. Rv1410 was originally postulated to function as a drug efflux pump, ...

WILEY2019

Evaluation of Auramine O staining and conventional PCR for leprosy diagnosis: A comparative cross-sectional study from Ethiopia

Stewart Cole, Charlotte Avanzi, Philippe Busso

Background Diagnosis of leprosy mainly relies on clinical examination due to the inconsistent sensitivity and poor reproducibility of the current laboratory tests. Utilisation of alternative methods to the standard Ziehl Neelsen (ZN), Fite-Faraco (FF) and ...

PUBLIC LIBRARY SCIENCE2018

Microfluidics-based immunofluorescence for fast staining of ALK in lung adenocarcinoma

Diego Gabriel Dupouy, Benjamin Pelz, Saska Brajkovic

BackgroundAnaplastic lymphoma kinase (ALK) is a key oncogenic driver in lung adenocarcinoma patients and its fusion proteins are routinely assessed. The microfluidic tissue processor (MTP) device is based on a chip-confined low-volume technology allowing f ...

BMC2018

An Amidase_3 domain-containing N-acetylmuramyl-L-alanine amidase is required for mycobacterial cell division

Neeraj Dhar, Ashima Bhaskar, Dong Li

Mycobacteria possess a multi-layered cell wall that requires extensive remodelling during cell division. We investigated the role of an amidase_3 domain-containing N-acetylmuramyl-L-alanine amidase, a peptidoglycan remodelling enzyme implicated in cell div ...

Nature Publishing Group2017

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Acid-fastness

A general strategy to develop fluorogenic polymethine dyes for bioimaging

Development and validation of a multiplex real-time qPCR assay using GMP-grade reagents for leprosy diagnosis

FasR Regulates Fatty Acid Biosynthesis and Is Essential for Virulence of Mycobacterium tuberculosis

Mass Spectrometry Methods for Bacterial Infection Diagnosis and Cancer Analysis

Infrared nanospectroscopic mapping of a single metaphase chromosome

Increased drug permeability of a stiffened mycobacterial outer membrane in cells lacking MFS transporter Rv1410 and lipoprotein LprG

Evaluation of Auramine O staining and conventional PCR for leprosy diagnosis: A comparative cross-sectional study from Ethiopia

Microfluidics-based immunofluorescence for fast staining of ALK in lung adenocarcinoma

Preserving Perceptual Contrast in Decolorization with Optimized Color Orders

An Amidase_3 domain-containing N-acetylmuramyl-L-alanine amidase is required for mycobacterial cell division

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A general strategy to develop fluorogenic polymethine dyes for bioimaging

Preserving Perceptual Contrast in Decolorization with Optimized Color Orders

Increased drug permeability of a stiffened mycobacterial outer membrane in cells lacking MFS transporter Rv1410 and lipoprotein LprG

FasR Regulates Fatty Acid Biosynthesis and Is Essential for Virulence of Mycobacterium tuberculosis

An Amidase_3 domain-containing N-acetylmuramyl-L-alanine amidase is required for mycobacterial cell division

Mass Spectrometry Methods for Bacterial Infection Diagnosis and Cancer Analysis

Evaluation of Auramine O staining and conventional PCR for leprosy diagnosis: A comparative cross-sectional study from Ethiopia

Infrared nanospectroscopic mapping of a single metaphase chromosome

Microfluidics-based immunofluorescence for fast staining of ALK in lung adenocarcinoma