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In vitro transcription and translation reactions have become popular for a bottom-up approach to synthetic biology. Concentrations of the mRNA intermediate are rarely determined, although knowledge of synthesis and degradation rates could facilitate ration ...
The four-electron reduction of oxygen by tetrathiafulvalene (TTF) in acidified 1,2-dichloroethane and at the acidified water/1,2-dichloroethane interface has been observed. Spectroscopy and ion transfer voltammetry results suggest that the reaction proceed ...
The septation initiation network (SIN) in Schizosaccharomyces pombe regulates cytokinesis. Cdc7p encodes the first kinase in the core SIN pathway. In this study, I performed a genetic screen to identify novel regulators of the SIN pathway by isolating spon ...
Expression of nestin has been suggested to be a characteristic of pancreatic islet stem cells. To determine whether nestin is indeed expressed in such putative cells during embryonic development, or in the adult pancreas after injury, we performed a cell l ...
Microtubules assume a variety of structures throughout the different stages of the cell cycle. Ensuring the correct assembly of such structures is essential to guarantee cell division. During mitosis, it is well established that the spindle assembly checkp ...
TiO2 nanoparticles (NPs) activated by UV irradiation are known to have a bactericidal effect. In this study we report the details of TiO2 NPs influence on the colony-forming capacity of E. coli in the dark at pH 4.0-4.5. At this pH the bacterial cells are ...
Cell-penetrating peptides (CPPs), such as the HIV TAT peptide, are able to translocate across cellular membranes efficiently. A number of mechanisms, from direct entry to various endocytotic mechanisms (both receptor independent and receptor dependent), ha ...
Coronaviruses (CoV), including SARS and mouse hepatitis virus (MHV), are enveloped RNA viruses that induce formation of double-membrane vesicles (DMVs) and target their replication and transcription complexes (RTCs) on the DMV-limiting membranes. The DMV b ...
Modulation of the microtubule and the actin cytoskeleton is crucial for proper cell division. Protein phosphorylation is known to be an important regulatory mechanism modulating these cytoskeletal networks. By contrast, there is a relative paucity of infor ...
This thesis presents, develops and applies new methodologies that can quantitatively probe the mechanical and viscoelastic properties of biomaterials at the nanoscale. Elastic and viscous properties are important characteristics of materials, such as many ...
