Concept
Recombinant DNA
Related publications (214)
Two strategies to improve transient gene expression in HEK293E and/or CHO-DG44 cells
Transient gene expression (TGE) is based on episomal DNA expression. This technique is used for the production of recombinant proteins that can be used as therapeutics. TGE is for example extremely useful for shortening the time between candidate proteins ...
2011Gene Delivery with Hyperbranched Polylysine
The delivery of exogenous nucleic acids into mammalian cells is a valuable technique for basic research studies on the expression, regulation and function of genes and proteins. At the same time, gene delivery to cultivated mammalian cells has become funda ...
EPFL2011The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability
Florian Maria Wurm, David Hacker, Lucia Baldi Unser
Generating stable, high-producing mammalian cell lines is a major bottleneck in the manufacture of recombinant therapeutic proteins. Conventional gene transfer methods for cell line generation rely on random plasmid integration, resulting in unpredictable ...
Wiley-Blackwell2011Efficient and reproducible mammalian cell bioprocesses without probes and controllers?
Florian Maria Wurm, Mohamed Farhat, David Hacker, Lucia Baldi Unser, Matthieu Dreyer, Stéphanie Tissot, Martino Reclari, Agata Oberbek
Bioprocesses for recombinant protein production with mammalian cells are typically controlled for several physicochemical parameters including the pH and dissolved oxygen concentration (DO) of the culture medium. Here we studied whether these controls are ...
Elsevier2011OrbShake Bioreactors for Mammalian Cell Cultures
Most of the biopharmaceuticals nowadays are produced by animal cells. The worldwide demand for a specific group of biopharmaceuticals, the monoclonal antibodies (mAbs), has significantly increased this last decade. The mAbs are used as therapeutics in part ...
EPFL2011Recombinant expression and functional characterization of the mouse olfactory receptor mOR256-17 in mammalian cells
Horst Vogel, Horst Pick, Luigino Grasso, Thamani Dahoun
Olfactory receptors (ORs) constitute the largest family of sensory membrane proteins in mammals. They play a key role within the olfactory system in recognizing and discriminating a nearly unlimited number of structurally diverse odorous molecules. The mol ...
2011Manufacturing recombinant proteins in kg-ton quantities using animal cells in bioreactors
Mammalian cells in bioreactors as production host are the focus of this review. We wish to briefly describe today's technical status and to highlight emerging trends in the manufacture of recombinant therapeutic proteins, focusing on Chinese hamster ovary ...
Elsevier2011Efficient Orthogonal Bioconjugation of Dendrimers for Synthesis of Bioactive Nanoparticles
Nanoparticles carrying biologically active functional sets (e.g., targeting moiety, payload, tracer) have potential use in a wide range of clinical applications. Though complex, such constructions should, as far as possible, have a defined molecular archit ...
2011Production of a thermostable fumarase in Saccharomyces cerevisiae for the bioconversion of fumarate to L-malate
The gene encoding fumarase (fum) from Thermus thermophilus was expressed in yeast Saccharomyces cerevisiae. The recombinant cells were heated at 70°C to inactivate indigenous enzymes and used for the bioconversion of fumaric acid to L-malic acid. By heatin ...
2011Tightening of DNA knots by supercoiling facilitates their unknotting by type II DNA topoisomerases
Giovanni Dietler, Andrzej Stasiak, Guillaume Witz
Using numerical simulations, we compare properties of knotted DNA molecules that are either torsionally relaxed or supercoiled. We observe that DNA supercoiling tightens knotted portions of DNA molecules and accentuates the difference in curvature between ...
2011