Concept

Green fluorescent protein

Related publications (460)

Sources, Occurrence and Characteristics of Fluorescent Biological Aerosol Particles Measured over the Pristine Southern Ocean

Julia Schmale, Andrea Baccarini, Alireza Moallemi, Robin Lewis Modini, Sebastian Johannes Heinz-Josef Landwehr

In this study we investigate the occurrence of primary biological aerosol particles (PBAP) over all sectors of the Southern Ocean (SO) based on a 90-day dataset collected during the Antarctic Circumnavigation Expedition (ACE) in austral summer 2016-2017. S ...

2021

Optical diffraction tomography from single-molecule localization microscopy

Michaël Unser, Thanh-An Michel Pham, Emmanuel Emilien Louis Soubies, Ferréol Arnaud Marie Soulez

Single-molecule localization microscopy (SMLM) is a powerful method for the imaging of cellular structures. This modality delivers nanoscale resolution by sequentially activating a subset of fluorescent molecules and by extracting their super-resolved posi ...

2021

Single-molecule localization microscopy

Suliana Manley, Juliette Griffie

Single-molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited microscopy techniques and can image biological structures at the molecular scal ...

SPRINGERNATURE2021

KAT Ligation for Rapid and Facile Covalent Attachment of Biomolecules to Surfaces

Nako Nakatsuka, Nicholas Spencer

The efficient and bioorthogonal chemical ligation reaction between potassium acyltrifluoroborates (KATs) and hydroxylamines (HAs) was used for the surface functionalization of a self-assembled monolayer (SAM) with biomolecules. An alkane thioether molecule ...

2021

An In Vivo Microfluidic Study of Bacterial Load Dynamics and Absorption in the C. elegans Intestine

Martinus Gijs, Thomas Lehnert, Vittorio Viri, Maël Arveiler

Caenorhabditiselegans (C. elegans) has gained importance as a model for studying host-microbiota interactions and bacterial infections related to human pathogens. Assessing the fate of ingested bacteria in the worm's intestine is therefore of great interes ...

MDPI2021

Alpha-Synuclein oligomerization and aggregation: All models are useful but only if we know what they model This is the reply to a comment "Alpha-synuclein oligomerization and aggregation: A model will always be a model" on the original article "Monitoring alpha-synuclein oligomerization and aggregation using bimolecular fluorescence complementation assays: What you see is not always what you get". The articles are accompanied by a Preface "How good are cellular models?".

Hilal Lashuel

Alpha-synuclein oligomerization is one of the early events on the pathway to Lewy body formation. Therefore, interfering with this process holds tremendous potential for developing therapies that block alpha-Syn pathology formation and toxicity. The develo ...

WILEY2021

A simple micropreparative gel electrophoresis technique for purification of proteins, nucleic acids, and bioconjugates

Ardemis Anoush Boghossian, Sayyed Hashem Sajjadi, Shang-Jung Wu, Vitalijs Zubkovs

The biochemical and biomedical fields hinge on the ability to effectively separate and purify biological macromolecules. Though this need is largely addressed with a variety of chromatographic and electrophoretic purification techniques, such techniques ar ...

2021

BET1 variants establish impaired vesicular transport as a cause for muscular dystrophy with epilepsy

Matteo Dal Peraro, Mykola Dergai, Ying Hu

BET1 is required, together with its SNARE complex partners GOSR2, SEC22b, and Syntaxin-5 for fusion of endoplasmic reticulum-derived vesicles with the ER-Golgi intermediate compartment (ERGIC) and the cis-Golgi. Here, we report three individuals, from two ...

WILEY2021

Anin vivomicrofluidic study of bacterial transit inC. elegansnematodes

Martinus Gijs, Thomas Lehnert, Matteo Cornaglia, Hüseyin Baris Atakan, Vittorio Viri

Caenorhabditis elegans(C. elegans) constitutes an important model organism for use in nutrition and aging studies. We report a novel method for studying the dynamics ofEscherichia coli(E. coli) bacterial transit through the worms' intestine. A microfluidic ...

2020

Chemogenetic Control of Nanobodies

Kai Johnsson, Julien Hiblot, Helen Rebecca Ostlund Farrants

Engineered nanobody allows reversible control of activity in cells through the binding of small molecules. We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling ...

NATURE PUBLISHING GROUP2020

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