Concept

Lactate dehydrogenase

Related publications (128)

Lactate metabolism shift in CHO cell culture: the role of mitochondrial oxidative activity

Florian Maria Wurm, Martin Jordan, Mathieu Benjamin Stettler, Francesca Zagari

Lactate production is monitored in industrial processes as a crucial metabolite for cultured mammalian cells. Typically lactate is strongly produced during the exponential growth phase, while its net consumption is frequently observed when cells enter into ...

Elsevier2013

Lactate brain metabolism in different mouse strains probed by hyperpolarized 13C MRS

Rolf Gruetter, Bernard Lanz, Mor-Miri Mishkovsky, Tian Cheng, Arnaud Comment, Josefina Adriana Maria Bastiaansen, Yuhei Takado

Lactate plays an important role in brain energetics and is essential for neuronal function. Alteration of its metabolism might be associated with mechanisms linked to neurodegenerative disorders1. The purpose of this study was to demonstrate the potential ...

2013

Cerebral extracellular lactate increase is predominantly nonischemic in patients with severe traumatic brain injury

Pierre Magistretti, Jocelyne Bloch, Reto Meuli

Growing evidence suggests that endogenous lactate is an important substrate for neurons. This study aimed to examine cerebral lactate metabolism and its relationship with brain perfusion in patients with severe traumatic brain injury (TBI). A prospective c ...

Nature Publishing Group2013

High expression of the aspartate–glutamate carrier Aralar1 favors lactate consumption in CHO cell culture

Florian Maria Wurm, Lucia Baldi Unser, Martin Jordan, Mathieu Benjamin Stettler, Francesca Zagari

Process performance of mammalian cell cultures can be strongly impacted by high lactate accumulation, which can be a clone or media-dependent characteristic. In this study, the expression of specific genes was measured in several Chinese hamster ovary cell ...

2013

Elucidating the role of neuron-astrocyte metabolic coupling in learning and memory

Monika Tadi

The brain has very high energy demands that are mainly met by the circulating blood glucose to ensure its proper functioning. Thus, it is not surprising that though the human brain weighs only 2- 3% of the body weight, it consumes approximately 25% of tota ...

EPFL2013

Net increase of lactate and glutamate concentration in activated human visual cortex detected with magnetic resonance spectroscopy at 7 tesla

Rolf Gruetter, Lijing Xin, Nicolas Kunz, Benoît Schaller

After the landmark studies reporting changes in the cerebral metabolic rate of glucose (CMR(Glc) ) in excess of those in oxygen (CMR(O2) ) during physiological stimulation, several studies have examined the fate of the extra carbon taken up by the brain, r ...

Wiley-Blackwell2013

In Vivo Magnetic Resonance Spectroscopy (MRS) of the Activated Human Brain at 7T

Benoît Schaller

The principal aim of this thesis was to investigate human brain energy metabolism during physiological activation using proton (1H) magnetic resonance spectroscopy (MRS) at 7 Tesla (T). High magnetic fields (≥ 7 T) provide several advantages in human brain ...

EPFL2013

Multidisciplinary Analysis of the Metabolic Shift to Lactate Consumption in CHO Cell Culture

Francesca Zagari

Mammalian cells represent the most widely used host system for the industrial production of recombinant therapeutic proteins. In order to increase productivity, chemically defined media are often used and optimized to provide the cells with the necessary n ...

EPFL2012

Cell metabolism monitoring using a microfluidic cartridge with enzyme-based microreactors

Nico de Rooij, Peter Van der Wal, Sara Talaei

We present a novel microfluidic approach for on-line analysis of cell metabolism by simultaneous measurement of two important indicators (glucose and lactate) in a small volume (100 µl) of cell-culture medium. The in-vitro study of cell metabolism is espec ...

2012

Sweet sixteen for ANLS

Pierre Magistretti, Luc Pellerin

Since its introduction 16 years ago, the astrocyte-neuron lactate shuttle (ANLS) model has profoundly modified our understanding of neuroenergetics by bringing a cellular and molecular resolution. Praised or disputed, the concept has never ceased to attrac ...

2012

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