Pine Island is a city in Goodhue and Olmsted counties in the U.S. state of Minnesota. Most of Pine Island is in Goodhue County, but a small part extends into Olmsted County, making that portion part of the Rochester metropolitan area. As of the 2020 census, the population was 3,769.
The community has a strong agricultural base, but has been transitioning over time into a bedroom community for nearby Rochester, which employs many local residents. Large development is planned for the Olmsted County side as it becomes a Rochester suburb, including the Elk Run Bioscience Park and Minnesota's first diverging diamond interchange.
Pine Island was platted in 1856. A post office has been in operation at Pine Island since 1856.
Pine Island lies along the Middle Fork of the Zumbro River. According to the United States Census Bureau, the city has an area of , of which is land and is water.
U.S. Highway 52 serves as a main route in the community.