Concept

Chimera (genetics)

Related publications (36)

Genetic engineering of embryonic stem cells

We describe a novel generation oflentiviral vectors that are particularly well suited for work with embryonic stem cells. The possibility of selecting cell lines with antibiotics and the rapid insertion of any combination of promoters and genes of interest ...

Swiss Medical Publishers Ltd / EMH Swiss Medical Publishers2007

Telomere dysfunction induces environmental alterations limiting hematopoietic stem cell function and engraftment

Maike Jaworski

Cell-intrinsic checkpoints limit the proliferative capacity of primary cells in response to telomere dysfunction. It is not known, however, whether telomere dysfunction contributes to cell-extrinsic alterations that impair stem cell function and organ home ...

2007

Rapid generation of stable transgenic embryonic stem cell lines using modular lentivectors

David Michael Suter

Generation of stable transgenic embryonic stem (ES) cell lines by classic transfection is still a difficult task, requiring time-consuming clonal selection, and hampered by clonal artifacts and gene silencing. Here we describe a novel system that allows co ...

2006

In vivo protein labeling for structural and functional investigation of the 5-HT3A neurotransmitter receptor

Erwin Ilegems

In this thesis, two different fluorescent labeling techniques for in vivo investigations on the 5-HT3 receptor (5-HT3R) functions are presented. This plasma membrane protein contains five subunits surrounding an ion channel that opens after binding of a 5- ...

EPFL2004

Signals from lateral plate mesoderm instruct endoderm toward a pancreatic fate

Anne Grapin-Botton, Mithlesh Kumar

During embryonic development, organs arise along the gut tube as a series of buds in a stereotyped anterior-posterior (A-P) pattern. Using chick-quail chimeras and in vitro tissue recombination, we studied the interactions governing the. induction and main ...

2003

S-phase synchronized CHO cells show elevated transfection efficiency and expression using CaPi

Florian Maria Wurm, Martin Jordan, Frédéric Grosjean

Various methods exist to transfect mammalian cells in culture. It is generally accepted that individual methods have to be optimized for each of the cell lines or cell types used. Despitethe use of optimized protocols, significant day-to-day variationsin t ...

2002

E2Fs regulate adipocyte differentiation

Johan Auwerx

When preadipocytes reenter the cell cycle, PPAR gamma expression is induced, coincident with an increase in DNA synthesis, suggesting the involvement of the E2F family of cell cycle regulators. We show here that E2F1 induces PPAR gamma transcription during ...

2002

Spindle positioning during the asymmetric first cell division of Caenorhabditis elegans embryos

Pierre Gönczy

Cell division during development in many cases generates daughter cells that differ not only in fate, but also in size. We investigate the mechanisms that ensure proper spindle positioning during such asymmetric divisions using the one-cell stage Caenorhab ...

2001

Tissue-specific requirements for the proprotein convertase furin/SPC1 during embryonic turning and heart looping

Daniel Constam

Furin, the mammalian prototype of a family of serine proteases, is required for ventral closure and axial rotation, and formation of the yolk sac vasculature. Here we show additionally that left-sided expression of pitx2 and lefty-2 are also perturbed in F ...

2000

Lentivirus vector gene expression during ES cell-derived hematopoietic development in vitro

Didier Trono, Romain Zufferey

The murine embryonal stem (ES) cell virus (MESV) can express transgenes from the long terminal repeat (LTR) promoter/enhancer in undifferentiated ES cells, but expression is turned off upon differentiation to embryoid bodies (EBs) and hematopoietic cells i ...

2000

Graph Chatbot

Chat with Graph Search

Ask any question about EPFL courses, lectures, exercises, research, news, etc. or try the example questions below.

DISCLAIMER: The Graph Chatbot is not programmed to provide explicit or categorical answers to your questions. Rather, it transforms your questions into API requests that are distributed across the various IT services officially administered by EPFL. Its purpose is solely to collect and recommend relevant references to content that you can explore to help you answer your questions.