Related publications (8)

C2CAplus: A One-Pot Isothermal Circle-to-Circle DNA Amplification System

Sebastian Maerkl, Laura Sophie Grasemann

Rolling circle amplification (RCA) is a widely used DNA amplification method that uses circular template DNA as input and produces multimeric, linear single- or double-stranded DNA. Circle-to-circle amplification (C2CA) has further expanded this method by ...
Washington2023

Solving the mystery of the missing plasmids in seventh pandemic Vibrio cholerae strains

Melanie Blokesch

The pathogen responsible for the continuing seventh cholera pandemic typically lacks self-replicating plasmids. Genetics, cell biology and bioinformatics analyses have identified two DNA-defence systems that protect bacterial populations from plasmids and ...
2022

Alfalfa leaf curl virus is efficiently acquired by its aphid vector Aphis craccivora but inefficiently transmitted

Faustine Ambroisine Charline Ryckebusch

Alfalfa leaf curl virus (ALCV) is the first geminivirus for which aphid transmission was reported. Transmission by Aphis craccivora was determined previously to be highly specific and circulative. Using various complementary techniques, the transmission jo ...
MICROBIOLOGY SOC2021

Adeno-Associated Virus Activates an Innate Immune Response in Normal Human Cells but Not in Osteosarcoma Cells

Peter Martin Beard

Adeno-associated virus (AAV) is a small, DNA-containing dependovirus with promising potential as a gene delivery vehicle. Given the variety of applications of AAV-based vectors in the treatment of genetic disorders, numerous studies have focused on the imm ...
2011

Interplay of DNA supercoiling and catenation during the segregation of sister duplexes

Andrzej Stasiak, Guillaume Witz, Pablo Hernandez

The discrete regulation of supercoiling, catenation and knotting by DNA topoisomerases is well documented both in vivo and in vitro, but the interplay between them is still poorly understood. Here we studied DNA catenanes of bacterial plasmids arising as a ...
Oxford University Press2009

Recombinant Adeno-Associated viral vectors are deficient in provoking a DNA damage response

Peter Martin Beard, Michail Fragkos

Adeno-associated virus type 2 (AAV2) provokes a DNA damage response that mimics a stalled replication fork. We have previously shown that this response is dependent on ataxia telangiectasia-mutated and Rad3- related kinase and involves recruitment of DNA r ...
2008

Photodissociation of Alkyl Iodides in Helium Nanodroplets III: Recombination

Marcel Drabbels, Andreas Braun

The recombination of fragments resulting from the photodissociation of (fluorinated) alkyl iodides in helium nanodroplets at a wavelength of 266 nm has been investigated by means of ion imaging techniques. It is found that in the case of CH3I an appreciabl ...
2007

Identification and molecular genetic analysis of replication functions of the bacteriocinogenic plasmid pIP404 from Clostridium perfringens

Stewart Cole

The replication functions of the bacteriocinogenic plasmid pIP404, from Clostridium perfringens, were localized to a 2.8-kb EcoRI-EcoRV fragment by cloning into a vector deficient for replication in Bacillus subtilis. This fragment contains two genes, cop ...
1988

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