Concept

Protein microarray

Related publications (139)

Protein-Functionalized Polymer Brushes

Harm-Anton Klok, Kai Johnsson, Stefano Tugulu, India Christina Sielaff, Anke Arnold

A new strategy for the prepn. of protein-functionalized polymer brushes is reported, which is based on a combination of surface-initiated atom transfer radical polymn. (ATRP), p-nitrophenyl chloroformate activation of the surface hydroxyl groups, and subse ...

2005

Comparative analysis of genes downstream of the Hoxd cluster in developing digits and external genitalia

Denis Duboule

Mammalian Hox genes encode transcription factors that are crucial for proper morphogenesis along the various body axes. Despite their extensive structural and functional characterization, the nature of their target genes remains elusive. We have addressed ...

2005

Microarray analysis of VEGF-C responsive genes in human lymphatic endothelial cells

Melody Swartz, Carolyn Yong Pullin

Vascular endothelial growth factor-C (VEGF-C) is considered one of the most important factors influencing lymphatic endothelial cell biology. The goal of this work was to characterize the gene expression response by lymphatic endothelial cells (LECs) to VE ...

2005

On-Line Counting of Cysteine Residues in Peptides during Electrospray Ionization by Electrogenerated Tags and Their Application to Protein Identification

Hubert Girault, Niels Erik Lion, Christophe Roussel, Loïc Dayon, Michel Prudent

The electrochemically induced mass spectrometric tagging of cysteines by substituted hydroquinones was studied for peptides in a classical electrospray solvent (i.e., MeOH/H2O/AcOH 50/49/1). The tagging efficiency was tested with different hydroquinone com ...

2005

Identification of molecular apocrine breast tumours by microarray analysis

Cathrin Brisken, Darlene Goldstein, Maryse Fiche

Previous microarray studies on breast cancer identified multiple tumour classes, of which the most prominent, named luminal and basal, differ in expression of the oestrogen receptor alpha gene (ER). We report here the identification of a group of breast tu ...

2005

Surface-Plasmon-Mediated Single-Molecule Fluorescence Through a Thin Metallic Film

Noelia Laura Bocchio

We demonstrate that fluorescence of single molecules in the nanometric vicinity of a thin gold film can be effectively excited and detected through the film with an epi-illumination scanning confocal micro- scope. A full theoretical treatment of the fluore ...

American Physical Society2005

Hardware-software design of a smart sensor for fully-electronic DNA hybridization detection

Carlotta Guiducci

This paper describes the design of a smart sensor for label-free detection of DNA hybridization. The sensor is based on a direct electrical transduction principle: it measures impedance variation at the interface between a bio-functionalized electrode and ...

2005

Contrasting the excited-state dynamics of the photoactive yellow protein chromophore: Protein versus solvent environments

Majed Chergui, Goran Zgrablic, Stefan Haacke

Wavelength- and time-resolved fluorescence expts. have been performed on the photoactive yellow protein, the E46Q mutant, the hybrids of these proteins contg. a nonisomerizing "locked" chromophore, and the native and locked chromophores in aq. soln. The ...

2004

A novel Mixture Model Method for identification of differentially expressed genes from DNA microarray data

Ali Ajdari Rad, Maryam Zaheri

Background The main goal in analyzing microarray data is to determine the genes that are differentially expressed across two types of tissue samples or samples obtained under two experimental conditions. Mixture model method (MMM hereafter) is a nonparamet ...

2004

A model-based optimization framework for the inference on gene regulatory networks from DNA array data

Vassily Hatzimanikatis, Eleftherios Papoutsakis

Identification of the regulatory structures in genetic networks and the formulation of mechanistic models in the form of wiring diagrams is one of the significant objectives of expression profiling using DNA microarray technologies and it requires the deve ...

2004

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