Concept

Protein production

Related publications (160)

Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Sophie Nallet

Although respiratory syncytial virus (RSV) causes severe lower respiratory tract infection in infants and adults at risk, no RSV vaccine is currently available. In this report, efforts toward the generation of an RSV subunit vaccine using recombinant RSV f ...

2009

TsrE: a sulfur-transferase involved in various anaerobic respiration pathways ?

Christof Holliger, Julien Maillard, Laure Prat

TsrE, a 32kDa sulfur-transferase with two rhodanese domains, was identified as a result of its over-expression during anaerobic tetrachloroethene respiration by the obligate gram-positive anaerobe Desulfitobacterium hafniense. The tsrE gene is part of an o ...

2009

Mild hypothermia improves transient gene expression yields several fold in Chinese hamster ovary cells

Florian Maria Wurm, David Hacker, Stéphanie Tissot, Sarah Wulhfard, Sophie Bouchet, Jean Cevey

Large-scale transient gene expression (TGE) in mammalian cells is a rapid method to generate recombinant proteins, but the volumetric productivity for secreted proteins is still more than an order of magnitude lower than the yields typically achieved with ...

2008

Coexpression of acidic fibroblast growth factor enhances specific productivity and antibody titers in transiently transfected HEK293 cells

Florian Maria Wurm, Gaurav Backliwal, Sébastien Chenuet

Recombinant proteins are of great commercial and scientific interest. However, most current production methods using mammalian cells involve the time- and labor-intensive step of creating stable cell lines. Although production methods based on transient ge ...

2008

Development of high-titer transient gene expression processes for manufacturing recombinant proteins

Gaurav Backliwal

The market for recombinant therapeutic proteins (including antibodies) is estimated to be greater than $50-billion and has a compounded annual growth rate of around 20%. More than 50% of all approved processes for manufacturing recombinant proteins use mam ...

EPFL2008

Accelerating the development time of recombinant mammalian cell lines producing high titers of protein therapeutics

Sébastien Chenuet

The forthcoming arrival on the market of numerous protein therapeutics that require high clinical doses will foster the need for high-producing mammalian cell lines. Furthermore, pressures to reduce the development time for new therapeutics has meant more ...

EPFL2008

One-step refolding and purification of disulfide-containing proteins with a C-terminal MESNA thioester

Maartje Martina Cornelia Bastings

Background Expression systems based on self-cleavable intein domains allow the generation of recombinant proteins with a C-terminal thioester. This uniquely reactive C-terminus can be used in native chemical ligation reactions to introduce synthetic groups ...

BioMed Central2008

Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions

Florian Maria Wurm, Gaurav Backliwal, Sébastien Chenuet, Sarah Wulhfard

Transient transfection allows for fast production of recombinant proteins. However, the current bottlenecks in transient transfection are low titers and low specific productivity compared to stable cell lines. Here, we report an improved transient transfec ...

2008

Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection

Florian Maria Wurm, David Hacker, Madiha Derouazi, Sébastien Chenuet, Nicolas Jaccard

Transfection with polyethylenimine (PEI) was evaluated as a method for the generation of recombinant Chinese hamster ovary (CHO DG44) cell lines by direct comparison with calcium phosphate-DNA coprecipitation (CaPO4) using both green fluorescent protein (G ...

2008

An affinity oligonucleotide displacement strategy to purify ribonucleoprotein complexes applied to human telomerase

Joachim Lingner

Antisense oligonucleotides have been used to study the structure and function of small nuclear ribonucleoprotein (snRNP) complexes and were adapted and modified for the purification of a variety of RNPs. We describe methods for recombinant expression and r ...

Humana Press2008

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