Concept

Western blot

Related publications (95)

Revisiting the Urech Synthesis of Hydantoins: Direct Access to Enantiopure 1,5-Substituted Hydantoins Using Cyanobenziodoxolone

Jérôme Waser, Franck Le Vaillant, Nina Declas

A method for the synthesis of enantiopure 1,5-substituted hydantoins was developed using a hypervalent iodine cyanation reagent (cyanobenziodoxolone, CBX) as a source of electrophilic carbon. Starting from inexpensive commercially available enantiopure pro ...

2019

The pro-apoptotic effect of a Terpene-rich Annona cherimola leaf extract on leukemic cell lines

Rita Sarkis, Maria Younes

Background The edible fruit Annona cherimola has previously shown many nutritional and medicinal properties. The current study evaluates the anti-cancer and anti-proliferative properties of Annona cherimola ethanolic leaf extract (AELE) on Acute Myeloid Le ...

BMC2019

In contrast to conventional microfluidics, where liquids are typically manipulated within closed channels, open-space microfluidics has emerged as a new class of techniques that are well suited for ver-satile interaction with biological substrates. In part ...

EPFL2019

A molecular logic gate enables super-resolved imaging of intracellular lipid droplets

Pablo Rivera Fuentes, Adam Miklos Eördögh

Photoactivatable dyes enable single-mol. imaging in biol. Despite progress in the development of new fluorophores and labeling strategies, many cellular compartments remain difficult to image beyond the limit of diffraction in living cells. For example, li ...

2019

Characterization of the predicted membrane anchor of reductive dehalogenases

Julien Maillard, Lorenzo Cimmino, Marie Joanna Marie-Joseph Dannay

BACKGROUND Organohalide respiration (OHR) is a metabolic process that couples the reduction of halogenated organic compounds to energy conservation in a few phylogenetically diverse anaerobic bacteria. In this study, tetrachloroethene (= perchloroethene, P ...

2018

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BackgroundAnaplastic lymphoma kinase (ALK) is a key oncogenic driver in lung adenocarcinoma patients and its fusion proteins are routinely assessed. The microfluidic tissue processor (MTP) device is based on a chip-confined low-volume technology allowing f ...

BMC2018

We report here the development of coreactant-based electro-generated chemiluminescence (ECL) as a surface-confined microscopy to image single cells and their membrane proteins. Labeling the entire cell membrane allows one to demonstrate that, by contrast w ...

American Chemical Society2017

Disrupting Y-Box-Binding Protein 1 Function Using OSU-03012 Prevents Endometriosis Progression in In Vitro and In Vivo Models

The objective of the present study was to test the ability of OSU-03012 (2-amino-N-[4-[5-phenanthren-2-yl-3-(trifluoromethyl)pyrazol-1-yl]phenyl]acetamide), a novel and potent celecoxib-derivative, to impair endometriosis progression in in vitro and in viv ...

Sage Publications Inc2017

Blotting-free and lossless cryo-electron microscopy grid preparation from nanoliter-sized protein samples and single-cell extracts

Henning Paul-Julius Stahlberg, Anastasia Syntychaki

We present a sample preparation method for cryo-electron microscopy (cryo-EM) that requires only 320 nL of sample to prepare a cryo-EM grid, depending on the protocol used. The sample is applied and spread on the grid by a microcapillary. The procedure doe ...

Elsevier BV2017

Bioluminescent Antibodies for Point-of-Care Diagnostics

Kai Johnsson, Rudolf Griss, Alberto Schena, Qiuliyang Yu, Lin Xue

We introduce a general method to transform antibodies into ratiometric, bioluminescent sensor proteins for the no-wash quantification of analytes. Our approach is based on the genetic fusion of antibody fragments to NanoLuc luciferase and SNAP-tag, the lat ...

2017

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