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The glycoprotein IgM is the major antibody produced in the primary immune response to antigens, circulating in the serum both as a pentamer and a hexamer. Pentameric IgM has a single J chain, which is absent in the hexamer. The mu (heavy) chain of IgM has ...
American Society for Biochemistry and Molecular Biology2005
We studied the transduction of primary human B lymphocytes and myeloma cells with lentiviral vectors. In peripheral blood B cells that had been activated with helper T cells (murine thymoma EL-4 B5) and cytokines, multiply attenuated HIV-1-derived vectors ...
Plasma treatment of PET films was carried out under argon, followed by exposure to an oxygen atmosphere. The films underwent considerable changes in surface composition and morphology, as demonstrated by contact angle measurements, FTIR-ATR, AFM, and XPS. ...
Asynchronous and synchronous calcium oscillations occur in a variety of cells. A well-established pathway for intercellular communication is provided by gap junctions which connect adjacent cells and can mediate electrical and chemical coupling. Several ex ...
We present a comparative study of the nucleation and growth of pentacene monolayer islands in the submonolayer regime onto inorganic substrates of SiO2 and sapphire (Al2O3) and organic substrates of poly(methyl-metacrylate) ...
The isolation and sepn. of the mol. variants of monoclonal IgA from cell culture supernatants is possible using several filtration and ion-exchange chromatog. steps, followed by size-exclusion chromatog. for the actual sepn. of the mol. variants. The latte ...
Production of human monoclonal autoantibodies to glutamic acid decarboxylase Mr 65,000 (GAD65), characterization of their isotype, binding affinity, V region sequences and competition with autoantibodies in patients' sera is described. Lymphocytes from a p ...
Monoclonal IgA was produced under serum free conditions by a murine hybridoma cell line (ZAC3) in a hollow fiber, a continuous stirred tank and a fluidized bed reactor. Differences in the antibody adsorption to DEAE chromatog. matrixes, an essential step i ...
PAH (N-(4-aminobenzoyl)glycin) clearance measurements have been used for 50 years in clinical research for the determination of renal plasma flow. The quantitation of PAH in plasma or urine is generally performed by colorimetric method after diazotation re ...
Monoclonal IgA antibodies have been produced by a hybridoma cell line grown in different bioreactors using serum-contg. and protein-free media in both basal and fortified versions. The effects of culture conditions on IgA prodn. and quality were studied us ...