Concept

Silver staining

Related publications (36)

Immunohistochemistry for the MEPHISTO X-PEEM

Over almost 50 years of its development, the science of immunology has become an indispensable tool for the understanding of the histology of tissues. Antibodies are highly specific probes, so that tissue structure can be interpreted not just by morphologi ...

Amer Inst Physics2000

The 3.7 angstrom projection map of the glycerol facilitator GlpF: a variant of the aquaporin tetramer

Henning Paul-Julius Stahlberg, Andreas Engel

GlpF, the glycerol facilitator protein of Escherichia coli, is an archetypal member of the aquaporin superfamily. To assess its structure, recombinant histidine-tagged protein was overexpressed, solubilized in octylglucoside and purified to homogeneity. Ne ...

Wiley-Blackwell2000

Development of a bioprocess for murine dimeric IgA production

Urs von Stockar

Monoclonal IgA was produced under serum free conditions by a murine hybridoma cell line (ZAC3) in a hollow fiber, a continuous stirred tank and a fluidized bed reactor. Differences in the antibody adsorption to DEAE chromatog. matrixes, an essential step i ...

1998

The clustering of telomeres and colocalization with Rap1, Sir3, and Sir4 proteins in wild-type Saccharomyces cerevisiae

Thierry Laroche

We have developed a novel technique for combined immunofluorescence/in situ hybridization on fixed budding yeast cells that maintains the three-dimensional structure of the nucleus as monitored by focal sections of cells labeled with fluorescent probes and ...

1996

Expression of active iron regulatory factor from a full-length human cDNA by in vitro transcription/translation

Lukas Kühn

Iron regulatory factor (IRF), also called iron responsive element-binding protein (IRE-BP), is a cytoplasmic RNA-binding protein which regulates post-transcriptionally transferrin receptor mRNA stability and ferritin mRNA translation. By using the polymera ...

1992

Interferon enhances the expression of Fc gamma receptors

Michel Aguet

Murine T2D4 cells derived from a T cell hybrid line were incubated with partially purified or electrophoretically pure mouse interferon and tested for the expression of Fc gamma R as assessed by a) counting the number of cells forming rosettes with IgG-sen ...

1980

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