Concept

Protein–lipid interaction

Related publications (47)

Conformational changes due to membrane binding and channel formation by staphylococcal alpha-toxin

Conformational changes occurring upon membrane binding and subsequent insertion of staphylococcal alpha-toxin were studied using complementary spectroscopic techniques. Experimental conditions were established where binding could be uncoupled from membrane ...

1997

Chloroplast F0F1 ATP synthase imaged by atomic force microscopy

Henning Paul-Julius Stahlberg

The F0F1 ATP synthase of chloroplasts was imaged using atomic force microscopy (AFM) in contact mode under physiological conditions. Chloroplast (CF0F1) ATP synthases were reconstituted into liposomes, Liposomes were adsorbed on a mica surface where they s ...

Elsevier BV1997

Incorporation and antibody recognition of a lipid-anchored membrane protein in supported lipid layers

Horst Vogel, Martha Liley

The formation of supported lipid layers incorporating promastigote surface protease (PSP), a glycosylphosphatidylinositol-anchored protein, is investigated using surface plasmon resonance. Both hydrophilic and hydrophobic substrates are used for the format ...

1997

Formation and characterization of lipopeptide layers at interfaces for the molecular recognition of antibodies

Horst Vogel, Claus Duschl

A lipopeptide, carrying the antigenic peptide segment 135-154 of VP1, one of the capsid proteins of the picornavirus which causes foot-and-mouth disease in cattle, was investigated in lipid monolayers on the water surface and on hydrophobic solid supports. ...

1996

Structural characterisation and membrane insertion of M13 procoat, M13 coat and Pf3 coat proteins

Membrane proteins fulfill many central functions in the biological membrane. The insertion process of these proteins and their structure, which are intimately linked to their function, are not yet well understood. As a model we studied three proteins recon ...

EPFL1996

Thermodynamics of the Membrane Insertion Process of the M13 Procoat Protein, a Lipid Bilayer Traversing Protein Containing a Leader Sequence

Horst Vogel

For the first time, the std. free energy change, DG Deg, of a membrane-inserting protein with a leader sequence has been detd. exptl., using M13 procoat protein as an example. The partition coeff. for the distribution of the procoat protein between the aq. ...

1996

Template-assembled melittin: structural and functional characterization of a designed, synthetic channel-forming protein

Horst Vogel

Template-assembled proteins (TASPs) comprising 4 peptide blocks, each of either the natural melittin sequence (melittin-TASP) or of a truncated melittin sequence (amino acid 6-26, melittin6-26-TASP), C-terminally linked to a (linear or cyclic) 10-amino aci ...

1994

Oligomerization of the channel-forming toxin aerolysin precedes insertion into lipid bilayers

Françoise Gisou van der Goot Grunberg

Oligomerization is a necessary step in channel formation by the bacterial toxin aerolysin. We have identified a region of aerolysin containing two tryptophans which influence the ability of the protein to oligomerize. Changing the tryptophan at position 37 ...

1993

TEMPLATE-ASSEMBLED SYNTHETIC PROTEINS DESIGNED TO ADOPT A GLOBULAR, 4-HELIX BUNDLE CONFORMATION FORM IONIC CHANNELS IN LIPID BILAYERS

Template-assembled synthetic proteins (TASPs) designed to adopt globular, four-helix bundle structures form ion channels in lipid bilayers. The rationale behind this work is that a bundle of amphiphilic alpha-helices may constitute a functional pore-formin ...

1993

The membrane insertion of colicins

Françoise Gisou van der Goot Grunberg

Pore-forming toxins, such as colicin A, are water-soluble proteins that insert into lipid bilayers. The water-soluble structure of Colicin A is known at a high resolution and this review describes the kinetic and structural steps involved in its soluble-to ...

1992

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