Concept

Immunoprecipitation

Related publications (42)

Development of multiplexed single-molecule imaging techniques to study chromatin invasion dynamics of chromatin effector proteins

DNA-binding proteins physically interact with the DNA and directly affect genomic functions. The eukaryotic genome is compacted into chromatin, limiting the DNA access to nuclear factors. In this Ph.D. thesis, I explored the dynamic mechanisms, that allow ...

EPFL2023

Systematic screening of viral and human genetic variation identifies antiretroviral resistance and immune escape link

Jacques Fellay, Christian Axel Wandall Thorball

Background: Considering the remaining threat of drug-resistantmutations (DRMs) to antiretroviral treatment (ART) efficacy, we investigated how the selective pressure of human leukocyte antigen (HLA)-restricted cytotoxic T lymphocytes drives certain DRMs' e ...

ELIFE SCIENCES PUBLICATIONS LTD2021

Site-Specific Protein Conjugation onto Fluorescent Single-Walled Carbon Nanotubes

Ardemis Anoush Boghossian, Shang-Jung Wu, Vitalijs Zubkovs

Semiconducting single-walled carbon nanotubes (SWCNTs) are among the few photostable optical emitters that are ideal for sensing, imaging, drug delivery, and monitoring of protein activity. These applications often require strategies for immobilizing prote ...

2020

Integrated microfluidic tools for the characterization of protein/DNA interactions in vitro and in vivo

Riccardo Dainese

The specific interaction between DNA and proteins constitutes one of the crucial elements in the regulation of gene expression. This thesis focuses on the development and optimization of two microfluidic-based technologies called SMiLE-seq and FloChIP that ...

EPFL2019

Analytical Approaches for Monitoring DNA–Protein Interactions

Ardemis Anoush Boghossian, Shang-Jung Wu

DNA–protein interactions play a critical role in cellular regulation. We herein review existing analytical methods for investigating these interactions, highlighting methods such as chromatin immunoprecipitation and yeast-one-hybrid that are used to identi ...

2019

Development of microfluidic tools and methods for the biochemical characterization of scFv and Cas9 affinity reagents and their applications in molecular detection

Ekaterina Emilova Petrova

Recent progress in protein engineering, empowered by the rapidly evolving fields of synthetic biology and DNA synthesis, has enabled the generation of various custom-designed proteins. Their thorough analysis has led to applications in areas including medi ...

EPFL2018

Polyserotonin Nanoparticles as Multifunctional Materials for Biomedical Applications

Nako Nakatsuka

Serotonin-based nanoparticles represent a class of previously unexplored multifunctional nanoplatforms with potential biomedical applications. Serotonin, under basic conditions, self-assembles into monodisperse nanoparticles via autoxidation of serotonin m ...

2018

Multicolor single-particle reconstruction of protein complexes

Pierre Gönczy, Suliana Manley, Kyle Michael Douglass, Christian Sieben, Niccolo Banterle

Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reco ...

2018

Tandem affinity purification of AtTERT reveals putative interaction partners of plant telomerase in vivo

Jana Majerská

The life cycle of telomerase involves dynamic and complex interactions between proteins within multiple macromolecular networks. Elucidation of these associations is a key to understanding the regulation of telomerase under diverse physiological and pathol ...

Springer Wien2017

Integrating gene synthesis and microfluidic protein analysis for rapid protein engineering

Sebastian Maerkl, Bruno Emanuel Ferreira De Sousa Correia, Ekaterina Emilova Petrova, Matthew Christopher Blackburn

The capability to rapidly design proteins with novel functions will have a significant impact on medicine, biotechnology and synthetic biology. Synthetic genes are becoming a commodity, but integrated approaches have yet to be developed that take full adva ...

Oxford Univ Press2016

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