Photofermentation is the fermentative conversion of organic substrate to biohydrogen manifested by a diverse group of photosynthetic bacteria by a series of biochemical reactions involving three steps similar to anaerobic conversion. Photofermentation differs from dark fermentation because it only proceeds in the presence of light.
For example, photo-fermentation with Rhodobacter sphaeroides SH2C (or many other purple non-sulfur bacteria) can be employed to convert small molecular fatty acids into hydrogen and other products.
Phototropic bacteria produce hydrogen gas via photofermentation, where the hydrogen is sourced from organic compounds.
C6H12O6 + 6H2O ->[{hv}] 6CO2 + 12H2
Photolytic producers are similar to phototrophs, but source hydrogen from water molecules that are broken down as the organism interacts with light. Photolytic producers consist of algae and certain photosynthetic bacteria.
12H2O ->[{hv}] 12H2 + 6O2(algae)
CO + H2O ->[{hv}] H2 + CO2(photolytic bacteria)
Photofermentation via purple nonsulfur producing bacteria has been explored as a method for the production of biofuel. The natural fermentation product of these bacteria, hydrogen gas, can be harnessed as a natural gas energy source. Photofermentation via algae instead of bacteria is used for bioethanol production, among other liquid fuel alternatives.
The bacteria and their energy source are held in a bioreactor chamber that is impermeable to air and oxygen free. The proper temperature for the bacterial species is maintained in the bioreactor. The bacteria are sustained with a carbohydrate diet consisting of simple saccharide molecules. The carbohydrates are typically sourced from agricultural or forestry waste.
In addition to wild type forms of Rhodopseudomonas palustris, scientists have used genetically modified forms to produce hydrogen as well. Other explorations include expanding the bioreactor system to hold a combination of bacteria, algae or cyanobacteria. Ethanol production is performed by the algae Chlamydomonas reinhardtii, among other species, in cycling light and dark environments.
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Dark fermentation is the fermentative conversion of organic substrate to biohydrogen. It is a complex process manifested by diverse groups of bacteria, involving a series of biochemical reactions using three steps similar to anaerobic conversion. Dark fermentation differs from photofermentation in that it proceeds without the presence of light. Fermentative/hydrolytic microorganisms hydrolyze complex organic polymers to monomers which are further converted to a mixture of lower-molecular-weight organic acids and alcohols by obligatory producing acidogenic bacteria.
Fermentative hydrogen production is the fermentative conversion of organic substrates to H2. Hydrogen produced in this manner is often called biohydrogen. The conversion is effected by bacteria and protozoa, which employ enzymes. Fermentative hydrogen production is one of several anaerobic conversions. Dark fermentation reactions do not require light energy. These are capable of constantly producing hydrogen from organic compounds throughout the day and night. Typically these reactions are coupled to the formation of carbon dioxide or formate.
Microbial fuel cell (MFC) is a type of bioelectrochemical fuel cell system also known as micro fuel cell that generates electric current by diverting electrons produced from the microbial oxidation of reduced compounds (also known as fuel or electron donor) on the anode to oxidized compounds such as oxygen (also known as oxidizing agent or electron acceptor) on the cathode through an external electrical circuit. MFCs produce electricity by using the electrons derived from biochemical reactions catalyzed by bacteria.
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