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Related publications (55)
A Simple, Robust, and Low-Cost Method To Produce the PURE Cell Free System
Sebastian Maerkl, Barbora Lavickova
We demonstrate a simple, robust, and low-cost method for producing the PURE cell-free transcription translation system. Our OnePot PURE system achieved a protein synthesis yield of 156 peg/mL at a cost of 0.09 USD/ktL, leading to a 14-fold improvement in c ...
AMER CHEMICAL SOC2019Black Lipid Membranes: Challenges in Simultaneous Quantitative Characterization by Electrophysiology and Fluorescence Microscopy
Esther Amstad, Stefan Matile, Maria Tsemperouli
Horizontal black lipid membranes (BLMs) enable optical microscopy to be combined with the electrophysiological measurements for studying ion channels, peptide pores, and ionophores. However, a careful literature review reveals that simultaneous fluorescenc ...
AMER CHEMICAL SOC2019Detection of bacteria
Hubert Girault, Horst Pick, Andreas Stephan Lesch, Milica Jovic, Yingdi Zhu
A method for bacteria detection and the assessment of the antimicrobial susceptibility profile uses a bacterial viability assay based on combining a highly efficient affinity bacteria enrichment/ separation with an electrochemical redox interrogation measu ...
2019Glycosphingolipid metabolism in cell fate specification
Giovanni D'Angelo, Laura Capolupo, Jaipreet Singh Loomba
Glycosphingolipids (GSLs) are ubiquitous components of eukaryotic plasma membranes that consist of a ceramide backbone linked to a glycan moiety. Both the ceramide and the glycan parts of GSLs display structural variations that result in a remarkable reper ...
2018Short-Term Thermal Acclimation Modifies the Metabolic Condition of the Coral Holobiont
Anders Meibom, Thomas Krüger, Emma Mary Gibbin, Julia Marie Jeanne Yvonne Bodin
The nutritional symbiosis between coral hosts and photosynthetic dinoflagellates is fundamental to the functioning of coral reefs. Rising seawater temperatures destabilize this relationship, resulting in drastic declines in world-wide coral cover. Thermal ...
2018The double-edged toxins
Many insect species are associated with endosymbiotic bacteria which have the particularity of living within host tissues. Endosymbionts benefit from this stable and nutritious environment, while providing ecological advantages to their host, such as prote ...
EPFL2018Towards artificial methanogenesis: biosynthesis of the [Fe]-hydrogenase cofactor and characterization of the semisynthetic hydrogenase
The greenhouse gas and energy carrier methane is produced on Earth mainly by methanogenic archaea. In the hydrogenotrophic methanogenic pathway the reduction of one CO2 to one methane molecule requires four molecules of H-2 containing eight electrons. Four ...
Royal Soc Chemistry2017Evolution of an intricate J-protein network driving protein disaggregation in eukaryotes
Paolo De Los Rios, Alessandro Barducci, Duccio Malinverni
Hsp70 participates in a broad spectrum of protein folding processes extending from nascent chain folding to protein disaggregation. This versatility in function is achieved through a diverse family of J-protein cochaperones that select substrates for Hsp70 ...
Elife Sciences Publications Ltd2017Archaebacteria in bioactive animal feed, method of making the composition and methods employing the composition
Duncan-Bruce Sutherland, Mario Michael Zaiss
The invention relates to a food supplement comprising or consisting of Archaebacteria, and particularly methanogenic Archaebacteria, to be used as a probiotic adjunct for animal feed. The supplement can be provided to e.g. farmed animals in addition to sta ...
2016Chemometric Analysis of Bacterial Peptidoglycan Reveals Atypical Modifications That Empower the Cell Wall against Predatory Enzymes and Fly Innate Immunity
Peptidoglycan is a fundamental structure for most bacteria. It contributes to the cell morphology and provides cell wall integrity against environmental insults. While several studies have reported a significant degree of variability in the chemical compos ...
American Chemical Society2016