Freshwater biology is the scientific biological study of freshwater ecosystems and is a branch of limnology. This field seeks to understand the relationships between living organisms in their physical environment. These physical environments may include rivers, lakes, streams, ponds, lakes, reservoirs, or wetlands. Knowledge from this discipline is also widely used in industrial processes to make use of biological processes involved with sewage treatment and water purification. Water presence and flow is an essential aspect to species distribution and influences when and where species interact in freshwater environments. In the UK, the Freshwater Biological Association based near Windermere in Cumbria was one of the early institutions to research the biology of freshwater and promote the concepts of trophism in lakes and demonstrated the process of migration from oligotrophic water through mesotrophic to marsh. Freshwater biology is also used to study the effects of climate change and increased human impact on both aquatic systems and wider ecosystems. Freshwater organisms, vertebrates especially, appear to be at a higher extinction risk from climate change than terrestrial or marine organisms. Freshwater habitats support a wide variety of organisms with habitats including rivers, streams, lakes, ponds, and wetlands. Running water is a type of freshwater habitat that mainly consists of rivers and streams. Running, fast-moving waters have a higher oxygen content, allowing different species to thrive and making pollution easier to combat. Running water is an open system, meaning it is not isolate and exchanges matter and energy with other systems. Being an open system, a lot of organic matter found in running water is from land runoff or further sediment upstream and this matter is an important source of food for many species. Flowing bodies of water begin at the headwaters, which include springs, lakes, and snowmelt, and travel to their mouths, typically another moving water channel or the ocean.

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