Concept

Coding region

Related publications (224)

Genotypic features of lentivirus transgenic mice

Didier Trono, Denis Duboule, Felix Naef, Frédéric Schütz, Marc-Olivier Sauvain

Lentivector-mediated transgenesis is increasingly used, whether for basic studies as an alternative to pronuclear injection of naked DNA or to test candidate gene therapy vectors. In an effort to characterize the genetic features of this approach, we first ...

American Society for Microbiology2008

Mild hypothermia improves transient gene expression yields several fold in Chinese hamster ovary cells

Florian Maria Wurm, David Hacker, Stéphanie Tissot, Sarah Wulhfard, Sophie Bouchet, Jean Cevey

Large-scale transient gene expression (TGE) in mammalian cells is a rapid method to generate recombinant proteins, but the volumetric productivity for secreted proteins is still more than an order of magnitude lower than the yields typically achieved with ...

2008

Vertebrate conserved non coding DNA regions have a high persistence length and a short persistence time

Philipp Bucher

ABSTRACT: BACKGROUND: The comparison of complete genomes has revealed surprisingly large numbers of conserved non-protein-coding (CNC) DNA regions. However, the biological function of CNC remains elusive. CNC differ in two aspects from conserved protein-co ...

2007

Genetic diversity of cyanobacterial communities in Lake Kinneret (Israel) using 16S rRNA gene, psbA and ntcA sequence analyses

The genetic diversity of cyanobacterial communities was studied at various depths in Lake Kinneret (Israel). Denaturing gradient gel electrophoresis (DGGE) of specific 16S rRNA gene PCR products showed significant differences in the cyanobacterial communit ...

2007

Molecular mechanism of hepcidin deficiency in a patient with juvenile hemochromatosis

Didier Trono, Bastien Mangeat

We describe a point mutation creating an additional ATG codon in the 5' untranslated region (UTR) of the HAMP gene, in a patient with juvenile hemochromatosis. By transient in vitro transfection studies, we provide evidence that the additional ATG is funct ...

Pensiero Scientifico / Ferrata Storti Foundation2007

GIScience joining with molecular biology: a novel approach of detecting signatures of natural selection within the genome

Stéphane Joost

Even if many research projects in population genetics and conservation biology collect a quantity of spatially located biological samples, and despite its present predominance in Science and its direct application to concerns of public society (health, foo ...

2007

Destabilization of interleukin-6 mRNA requires a putative RNA stem-loop structure, an AU-rich element, and the RNA-binding protein AUF1

Lukas Kühn, Barbara Grisoni-Neupert

Interleukin-6 mRNA is unstable and degraded with a half-life of 30 min. Instability determinants can entirely be attributed to the 3' untranslated region. By grafting segments of this region to stable green fluorescent protein mRNA and subsequent scanning ...

2006

The Ser/Thr protein kinase PknB is essential for sustaining mycobacterial growth

Stewart Cole, Pablo Fernandez

The receptor-like protein kinase PknB from Mycobacterium tuberculosis is encoded by the distal gene in a highly conserved operon, present in all actinobacteria, that may control cell shape and cell division. Genes coding for a PknB-like protein kinase are ...

2006

C/EBPbeta couples dopamine signalling to substance P precursor gene expression in striatal neurones

Pierre Magistretti, Jean-René Cardinaux

Dopamine-induced changes in striatal gene expression are thought to play an important role in drug addiction and compulsive behaviour. In this study we report that dopamine induces the expression of the transcription factor CCAAT/Enhancer Binding Protein b ...

2006

International Union of Pharmacology. LXI. Peroxisome proliferator-activated receptors.

Johan Auwerx, Walter Wahli, Liliane Michalik

The three peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors of the nuclear hormone receptor superfamily. They share a high degree of structural homology with all members of the superfamily, particularly in the D ...

2006

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