Concept

Two-hybrid screening

Related publications (96)

Droplet-Based Microfluidic Platforms for the Encapsulation and Screening of Mammalian Cells and Multicellular Organisms

High-throughput, cell-based assays require small sample volumes to reduce assay costs and to allow for rapid sample manipulation. However, further miniaturization of conventional microtiter plate technology is problematic due to evaporation and capillary a ...

2008

Labelling of fusion proteins with synthetic probes

Kai Johnsson, Alexandre Juillerat

The invention relates to new proteins called alkylcytosine transferases (ACTs) derived from O-alkylguanine-DNA alkyltransferase, and to substrates for ACTs specifically transferring a label to these ACTs and to fusion proteins comprising these. The subs ...

2008

Matrix and Steiner-triple-system smart pooling assays for high-performance transcription regulatory network mapping

Bart Deplancke

Yeast one-hybrid (Y1H) assays provide a gene-centered method for the identification of interactions between gene promoters and regulatory transcription factors (TFs). To date, Y1H assays have involved library screens that are relatively expensive and labor ...

2007

Hybrid identification methods for the reconstruction of genetic regulatory networks

Giancarlo Ferrari Trecate

This tutorial paper considers the problem of reconstructing Genetic Regulatory Networks (GRNs) from gene expression measurements. Among various modeling frameworks that have been proposed for these biological systems, we focus on PieceWise Affine (PWA) mod ...

2007

A protein linkage map of the ESAT-6 secretion system 1 (ESX-1) of Mycobacterium tuberculosis

Stewart Cole

Tuberculosis is a chronic infectious disease caused by bacteria of the Mycobacterium tuberculosis complex. One of the major contributors to virulence and intercellular spread of M. tuberculosis is the ESAT-6 secretion system 1 (ESX-1) that has been lost by ...

2007

Gateway-Compatible Yeast One-Hybrid Screens

Bart Deplancke

Protein-DNA interactions (PDIs) between transcription factors (TFs) and their target genes form the backbone of transcription regulatory networks. Such PDIs can be identified with either a TF or a gene as a starting point. The Gateway-compatible yeast one- ...

2006

Generation of recombinant Chinese hamster ovary cell lines by microinjection

Florian Maria Wurm, Madiha Derouazi, Martin Jordan, Philippe Pierre Girard

Microinjection is a gene transfer technique enabling partial control of plasmid delivery into the nucleus or cytoplasm of cultured animal cells. Here this method was used to establish various recombinant mammalian cell lines. The injection volume was estim ...

Kluwer Academic Publishers, Springer2006

Protein function microarrays based on self-immobilizing and self-labeling fusion proteins

Harm-Anton Klok, Kai Johnsson, Stefano Tugulu, India Christina Sielaff, Anke Arnold

Protein microarrays are an attractive approach for the high-throughput analysis of protein function, but their impact on proteomics has been limited by the technical difficulties associated with their generation. Here we demonstrate that fusion proteins of ...

2006

High throughput one-hybrid system

Bart Deplancke

The invention relates to a new high throughput yeast one-hybrid screening system ...

2005

Method for identification of suitable fragmentation sites in a reporter protein

Kai Johnsson, Petra Tafelmeyer

The invention concerns a combinatorial method for the generation of new split-protein sensors, and its application towards the (beta/alpha)8-barrel enzyme N-(5'-phosphoribosyl)-anthranilate isomerase Trp1p from Saccharomyces cerevisiae is demonstrated. The ...

2005

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