Concept

Two-hybrid screening

Related publications (96)

Synthese und Paarungseigenschaften von pDNA und deren Anwendung bei der Entwicklung funktionalisierter Ribozyme

Within this project methods for the preparation of the unnatural nucleic acids 3'-O-Methyl-pRNA and pDNA (3'-Desoxy-pRNA) were developed. The pairing properties of these two systems have been examined and compared with that of pRNA. All of the three system ...

EPFL2004

A general method for the covalent labeling of fusion proteins with small molecules in vivo

Horst Vogel, Kai Johnsson, Horst Pick, Thomas Gronemeyer, Antje Keppler

Characterizing the movement, interactions, and chem. microenvironment of a protein inside the living cell is crucial to a detailed understanding of its function. Most strategies aimed at realizing this objective are based on genetically fusing the protein ...

2003

Transfection of partially purified plasmid DNA for high level transient protein expression in HEK293-EBNA cells

Florian Maria Wurm, Martin Jordan, Jason Wright

One of the major constraints to performing large-scale transfections of cultured mammalian cells for the transient expression of recombinant proteins is the production of large quantities of purified plasmid DNA. In this report partially purified plasmid D ...

2003

Balancing GFP reporter plasmid quantity in large-scale transient transfections for recombinant anti-human Rhesus-D IgG1 synthesis

Horst Vogel, Florian Maria Wurm, Horst Pick

Using transient expression, high amounts (>20 mg/mL) of secreted anti-human Rhesus-D IgG1 were produced in a suspension-adapted HEK293 EBNA cell line (Meissner et al., Biotechnol Bioeng 75: 197-203, 2001). Time of harvest was 3 days after transfection. For ...

2002

The small heterodimer partner interacts with the liver X receptor alpha and represses its transcriptional activity

Johan Auwerx, Kristina Schoonjans

The small heterodimer partner SHP (NR0B2) is an unusual nuclear receptor that lacks the typical DNA binding domain common to most nuclear receptors. SHP has been reported to act as a corepressor for several nuclear receptors, but its exact mechanism of act ...

Endocrine Society2002

Transient gene expression: recombinant protein production with suspension-adapted HEK293-EBNA cells

Florian Maria Wurm, Horst Pick, Klaus Friedrich

Transient gene expression (TGE) in mammalian cells at the reactor scale is becoming increasingly important for the rapid production of recombinant proteins. We improved a process for transient calcium phosphate-based transfection of HEK293-EBNA cells in a ...

2001

Inter- and intramolecular domain interactions of the catalase-peroxidase KatG from M. tuberculosis

Kai Johnsson, Martin Wilming

The inter- and intramol. interactions between the different domains of the catalase-peroxidase KatG from Mycobacterium tuberculosis were analyzed using the two-hybrid assay. It was shown that the dimerization of the enzyme is due to a strong interaction of ...

2001

Too much interference: Injection of double-stranded RNA has nonspecific effects in the zebrafish embryo

Andrew Charles Oates

We have investigated the ability of double-stranded RNA (dsRNA) to inhibit gene expression in a vertebrate, the zebrafish, Danio rerio. Injection of dsRNA corresponding to the T-box gene tbx16/spadetail (spt) into early wild-type embryos caused a rapid and ...

2000

Olfaction in birds: differential embryonic expression of nine putative odorant receptor genes in the avian olfactory system

Igor Allaman

We have isolated nine putative odorant receptor genes from the chick, named COR1 to COR9, that belong to the large multigene family of olfactory G protein-coupled receptors found in the fish, rat, mouse, dog, and human. By combining genomic DNA blot analys ...

Elsevier1996

RAP1 stimulates single- to double-strand association of yeast telomeric DNA: implications for telomere-telomere interactions

Thierry Laroche

Repressor Activator Protein 1 (RAP1) of Saccharomyces cerevisiae is an abundant nuclear protein implicated in telomere length maintenance, transactivation, and in the establishment of silent chromatin domains. The RAP1 binding site 5' of the yeast HIS4 gen ...

1994

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