Concept

Two-photon excitation microscopy

Related publications (489)

Two-photon imaging through a multimode fiber

Demetri Psaltis, Christophe Moser, Edgar Emilio Morales Delgado

In this work we demonstrate 3D imaging using two-photon excitation through a 20 cm long multimode optical fiber (MMF) of 350 mu m diameter. The imaging principle is similar to single photon fluorescence through a MMF, except that a focused femtosecond puls ...

Optical Soc Amer2015

RGD-conjugated two-photon absorbing near-IR emitting fluorescent probes for tumor vasculature imaging

Alma Rosa Morales Morales, Xuan Liu

Observation of the activation and inhibition of angiogenesis processes is important in the progression of cancer. Application of targeting peptides, such as a small peptide that contains adjacent L-arginine (R), glycine (G) and L-aspartic acid (D) residues ...

Royal Society of Chemistry2015

Accounting for Limited Detection Efficiency and Localization Precision in Cluster Analysis in Single Molecule Localization Microscopy

Aleksandra Radenovic, Jayakrishnan Unnikrishnan, Arun Shivanandan

Single Molecule Localization Microscopy techniques like PhotoActivated Localization Microscopy, with their sub-diffraction limit spatial resolution, have been popularly used to characterize the spatial organization of membrane proteins, by means of quantit ...

Public Library of Science2015

PALMsiever: a tool to turn raw data into results for single-molecule localization microscopy

Suliana Manley, Seamus John Holden, Thomas Pengo

During the past decade, localization microscopy (LM) has transformed into an accessible, commercially available technique for life sciences. However, data processing can be challenging to the non-specialist and care is still needed to produce meaningful re ...

Oxford University Press2015

BigNeuron: Large-Scale 3D Neuron Reconstruction from Optical Microscopy Images

Sean Lewis Hill

Understanding the structure of single neurons is critical for understanding how they function within neural circuits. BigNeuron is a new community effort that combines modern bioimaging informatics, recent leaps in labeling and microscopy, and the widely r ...

Elsevier2015

A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue

Pablo Rivera Fuentes

Imaging mobile zinc in acidic environments remains challenging because most small-mol. optical probes display pH-dependent fluorescence. Here we report a reaction-based sensor that detects mobile zinc unambiguously at low pH. The sensor responds reversibly ...

2015

DprE1 Is a Vulnerable Tuberculosis Drug Target Due to Its Cell Wall Localization

Stewart Cole, Gaëlle Séraphine Kolly, Joao Pedro De Almeida Neres, Raju Mukherjee

The flavo-enzyme DprE1 catalyzes a key epimerization step in the decaprenyl-phosphoryl d-arabinose (DPA) pathway, which is essential for mycobacterial cell wall biogenesis and targeted by several new tuberculosis drug candidates. Here, using differential r ...

American Chemical Society2015

Digital confocal microscopy through a multimode fiber

Demetri Psaltis, Christophe Moser, Ioannis Papadopoulos, Damien Claude-Marie Loterie, Salma Farahi, Alexandre Goy

Confocal laser-scanning microscopy is a well-known optical imaging method where a pinhole is used in the illumination and detection pathways of a normal microscope, in order to selectively excite and detect a particular focal volume. The advantage of this ...

Spie-Int Soc Optical Engineering2015

High-Resolution Correlative Microscopy: Bridging the Gap between Single Molecule Localization Microscopy and Atomic Force Microscopy

Aleksandra Radenovic, Georg Fantner, Lely Feletti, Adrian Pascal Nievergelt, Pascal Damian Odermatt, Hendrik Georges Deschout, Arun Shivanandan, Radek Jankele

Nanoscale characterization of living samples has become essential for modern biology. Atomic force microscopy (AFM) creates topological images of fragile biological structures from biomolecules to living cells in aqueous environments. However, correlating ...

Amer Chemical Soc2015

Modeling enzymatic hydrolysis of lignocellulosic substrates using confocal fluorescence microscopy I: Filter paper cellulose

Jeremy Luterbacher

Enzymatic hydrolysis is one of the critical steps in depolymerizing lignocellulosic biomass into fermentable sugars for further upgrading into fuels and/or chemicals. However, many studies still rely on empirical trends to optimize enzymatic reactions. An ...

Wiley-Blackwell2015

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