Concept

Two-photon excitation microscopy

Related publications (489)

Single-molecule imaging of transcription factor binding to DNA in live mammalian cells

Imaging single fluorescent proteins in living mammalian cells is challenged by out-of-focus fluorescence excitation. To reduce out-of-focus fluorescence we developed reflected light-sheet microscopy (RLSM), a fluorescence microscopy method allowing selecti ...

Nature Publishing Group2013

Enhancement of Second Harmonic Signal in Nanofabricated Cones

Aleksandra Radenovic, Andrea Lovera, Oriol López Sánchez, Fabrizia Dutto, Martin Heiss

Geometrical effects in optical nanostructures on nanoscale can lead to interesting phenomena such as inhibition of spontaneous emission,(1,2) high-reflecting omnidirectional mirrors, structures that exhibit low-loss-waveguiding,(3) and light confinement.(4 ...

American Chemical Society2013

Digital holographic confocal microscope

Demetri Psaltis, Alexandre Goy

We demonstrate experimentally a scanning confocal microscopy technique based on digital holographic recording of the scanned spot. The data collected in this way contains all the information to produce three-dimensional images. Several methods to treat the ...

Spie-Int Soc Optical Engineering2013

Two-photon microscopy of the mouse cochlea in situ for cellular diagnosis

Demetri Psaltis, Ye Pu, Chia-Lung Hsieh, Xin Yang

Sensorineural hearing loss is the most common type of hearing loss worldwide, yet the underlying cause is typically unknown because the inner ear cannot be biopsied today without destroying hearing, and intracochlear cells have not been imaged with resolut ...

Spie-Soc Photo-Optical Instrumentation Engineers2013

BODIPY-phosphane as a versatile tool for easy access to new metal-based theranostics

Angela Casini, Olivier Pierre Zava

A new BODIPY-phosphane was synthesized and proved to be a versatile tool for imaging organometallic complexes. It also led to easy access to a new family of theranostics, featuring gold, ruthenium and osmium complexes. The compounds' cytotoxicity was teste ...

Royal Soc Chemistry2013

Morphological investigation of polylactide/microfibrillated cellulose composites

Pierre-Etienne Bourban, John Christopher Plummer, Carole Boissard

Optical microscopy and transmission electron microscopy have been used to investigate the morphology of polylactide (PLA)/microfibrillated cellulose (MFC) composites prepared by: compression molding of wet-comingled MFC and PLA latex or powder, twin-screw ...

Springer Berlin Heidelberg2013

Advanced Image Processing for Biology, and the Open Bio Image Alliance (OBIA)

Michaël Unser, Daniel Sage, Ricard Delgado Gonzalo

The field of biological imaging has evolved considerably during the past decade as a result of recent (r)evolutions in fluorescence labeling and optical microscopy. Bioimage informatics has been identified as a top priority to cope with the ever-increasing ...

EUSIPCO2013

Molecular Architecture of Plant Thylakoids under Physiological and Light Stress Conditions: A Study of Lipid-Light-Harvesting Complex II Model Membranes

Giovanni Dietler, Andrzej Kulik

In this study, we analyzed multibilayer lipid-protein membranes composed of the photosynthetic light-harvesting complex II (LHCII; isolated from spinach [Spinacia oleracea]) and the plant lipids monogalcatosyldiacylglycerol and digalactosyldiacylglycerol. ...

Amer Soc Plant Biologists2013

Can Localization Microscopy Benefit From Approximation Theory?

Michaël Unser, Cédric René Jean Vonesch, Hagay Kirshner

We introduce a general and computationally efficient approach to 3-D localization microscopy. The main idea is to construct a continuous-domain representation of the PSF by expanding it in a polynomial B-spline basis. This allows us to fit the PSF to the d ...

IEEE2013

Optimal antibunching in passive photonic devices based on coupled nonlinear resonators

Vincenzo Savona

We propose the use of weakly nonlinear passive materials for prospective applications in integrated quantum photonics. It is shown that strong enhancement of native optical nonlinearities by electromagnetic field confinement in photonic crystal resonators ...

Institute of Physics (IoP) and Deutsche Physikalische Gesellschaft2013

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