Concept

Plant tissue culture

Related publications (22)

Bipolar resistivity profiling of 3D tissue culture

Philippe Renaud, Yann Barrandon, Olof Pontus Linderholm, Jonas Vannod

We describe a new low-cost technique for continuous monitoring of the thickness of biofilms and tissue cultures, and we demonstrate the advantage of using electrodes of different dimensions to probe different depths of a sample. We have used electric imped ...

2007

In vitro breeding of Brassica juncea L. to enhance metal accumulation and extraction properties

Jean-Paul Schwitzguebel, Erika Nehnevajova

In vitro breeding and somaclonal variation were used as tools to improve the potential of Indian mustard (Brassica juncea L.) to extract and accumulate toxic metals. Calli from B. juncea were cultivated on a modified MS medium supplemented with 10–200 μM C ...

2007

Two-dimensional microimpedance imaging for cell culture monitoring

Olof Pontus Linderholm

This thesis presents the fabrication and design of an impedance imaging sensor, and its application to monitoring in-vitro skin culture. Three-dimensional tissue cultures are becoming increasingly important in biological and medical research. There is, how ...

EPFL2006

Two-dimensional impedance imaging of cell migration and epithelial stratification

Philippe Renaud, Yann Barrandon, Thomas Michael Braschler, Olof Pontus Linderholm, Jonas Vannod

We present a miniaturized impedance imaging system, developed for 2D imaging of cell and tissue culture. The system is based on 16 microelectrodes (5 µm × 4 mm). An equivalent circuit for four-point (tetrapolar) impedance spectra was developed and validate ...

2006

Nodal stability determines signaling range

Daniel Constam, Yixing Chen

Secreted TGFbeta proteins of the Nodal family pattern the vertebrate body axes and induce mesoderm and endoderm . Nodal proteins can act as morphogens , but the mechanisms regulating their activity and signaling range are poorly understood. In particular, ...

2005

Non-GMO approach for the improvement of heavy metal accumulation and extraction of high yielding crop species for efficient phytoextraction of contaminated soil

Erika Nehnevajova

Some plants able to take up heavy metals from contaminated soils offer a possibility to clean up sites contaminated with heavy metals. Plants thus act as a solar driven pump, which can extract and concentrate heavy metals from the environment. Since most o ...

EPFL2005

The Listeria monocytogenes lemA gene product is not required for intracellular infection or to activate fMIGWII-specific T cells

Clearance of the intracellular bacterial pathogen Listeria monocytogenes requires antigen-specific CD8(+) T cells. Recently it was shown that activation of class Ib major histocompatibility complex (MHC)-restricted CD8(+) T cells alone is sufficient for im ...

American Society for Microbiology2003

A three-dimensional multi-electrode array for multi-site stimulation and recording in acute brain slices

Philippe Renaud, Marc Olivier Heuschkel

Several multi-electrode array devices integrating planar metal electrodes were designed in the past 30 years for extracellular stimulation and recording from cultured neuronal cells and organotypic brain slices. However, these devices are not well suited f ...

2002

Biocompatibility of Si-based arrays of electrodes coupled to organotypic hippocampal brain slice cultures

In this study we examined the passive biocompatibility of a three-dimensional microelectrode array (MEA), designed to be coupled to organotypic brain slice cultures for multisite recording of electrophysiological signals. Hippocampal (and corticostriatal) ...

2001

Noradrenaline- and vasoactive intestinal peptide-containing neuronal systems in neocortex: functional convergence with contrasting morphology

Pierre Magistretti

Neurotransmitter-specific anatomical techniques have provided a tool to define the morphological constraints within which a given neurotransmitter will exert its cellular actions. Biochemical and electrophysiological approaches have revealed the nature of ...

1988

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