Concept
CRISPR
Related publications (62)
Development of multiplexed single-molecule imaging techniques to study chromatin invasion dynamics of chromatin effector proteins
DNA-binding proteins physically interact with the DNA and directly affect genomic functions. The eukaryotic genome is compacted into chromatin, limiting the DNA access to nuclear factors. In this Ph.D. thesis, I explored the dynamic mechanisms, that allow ...
EPFL2023Secondary structure of the human mitochondrial genome affects formation of deletions
Jacques Fellay, Konstantin Popadin
BackgroundAging in postmitotic tissues is associated with clonal expansion of somatic mitochondrial deletions, the origin of which is not well understood. Such deletions are often flanked by direct nucleotide repeats, but this alone does not fully explain ...
BMC2023Archaeal type IV pili stabilize Haloferax volcanii biofilms in flow
Alexandre Louis André Persat, Lorenzo Anton-Louis Talà, Pascal Damian Odermatt
Multicellular communities of contiguous cells attached to solid surfaces called biofilms represent a common microbial strategy to improve resilience in adverse environments.1-3 While bacterial biofilms have been under intense investigation, whether archaea ...
Cambridge2023RNA-based nanomedicines and their clinical applications
RNA-based nanomedicines encompass a range of therapeutic approaches that utilize RNA molecules or molecules that target RNAs for the treatment or prevention of diseases. These include antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), endo ...
Beijing2023Allosteric control of type I-A CRISPR-Cas3 complexes and establishment as effective nucleic acid detection and human genome editing tools
Henning Paul-Julius Stahlberg, Dongchun Ni
Type I CRISPR-Cas systems typically rely on a two-step process to degrade DNA. First, an RNA-guided complex named Cascade identifies the complementary DNA target. The helicase-nuclease fusion enzyme Cas3 is then recruited in trans for processive DNA degrad ...
CELL PRESS2022An optimized high throughput platform, from genetic transformation to fermentation, to screen for secreted laccases in Aspergillus niger
Laure Marie Colette Leynaud-Kieffer Curran
Many challenges are faced in the conversion of biomass into advanced biofuels, one of which is finding the correct organism for the job. The filamentous fungus Aspergillus niger has been chosen as a biocatalyst for cellulose, hemicellulose, and lignin degr ...
EPFL2022Towards automating de novo protein design for novel functionalities: controlling protein folds and protein-protein interactions
The sheer size of the protein sequence space is massive: a protein of 100 residues can have 20^100 possible sequence combinations; and knowing that this exceeds the number of atoms in the universe, the chance of randomly discovering a stable new sequence w ...
EPFL2022GCN2 adapts protein synthesis to scavenging-dependent growth
Pancreatic cancer cells with limited access to free amino acids can grow by scavenging extracellular protein. In a murine model of pancreatic cancer, we performed a genome-wide CRISPR screen for genes required for scavenging-dependent growth. The screen id ...
2022