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Information on the temporal order, the kinetics, and the energetics of each of the reaction intermediates is required in order to fully elucidate the mechanism of catalytic interaction of G proteins with their receptors. Intrinsic monitors, such as light s ...
The biocompatibility of microcapsules made by the co-acervation of alginate and poly(l-lysine) (PLL) was enhanced by coating the surface of these microcapsules with a poly(ethylene glycol) (PEG)-based hydrogel. The hydrogel was formed by an interfacial pho ...
Time-integrated fluorescence expts. on native bacteriorhodopsin and on its non-isomerizing form bR5.12 are reported. The exptl. set-up was designed such as to observe emission exclusively from the excited state intermediate I-460. We obtain the first syste ...
Absorption, fluorescence and excitation spectra of three-dimensional bacteriorhodopsin crystals harvested from a lipidic cubic phase are presented. The combination of the spectroscopic expts. performed at room temp., controlled pH and full external hydrati ...
A self-consistent discontinuum theory describing the dependence of the diffuse reflectance and diffuse transmittance of powders on their optical absorption coeff. is presented. The theory is valid for particle sizes large compared to the exciting wavelengt ...