Concept
Gastrulation
Related publications (115)
Engineering hydrogel microenvironments for epithelial organoid culture
Organoids, miniature tissues generated from self-organizing stem cells within three-dimensional (3D) extracellular matrices (ECM), have opened up exciting possibilities for in vitro studies of complex physiological processes. A key factor in the success of ...
EPFL2024Multimodal characterization of murine gastruloid development
Matthias Lütolf, Alexandre Gauthier Aurèle Mayran, Stefano Davide Vianello, Raphaël Ortiz
Gastruloids are 3D structures generated from pluripotent stem cells recapitulating fundamental principles of embryonic pattern formation. Using single-cell genomic analysis, we provide a resource mapping cell states and types during gastruloid development ...
CELL PRESS2023Structure and regulation of the myotonic dystrophy kinase-related Cdc42-binding kinase
Charlotte Julie Caroline Gehin
Protein kinases of the dystonia myotonica protein kinase (DMPK) family are critical regulators of actomyosin contractility in cells. The DMPK kinase MRCK1 is required for the activation of myosin, leading to the develop-ment of cortical tension, apical con ...
CELL PRESS2023An automated do-it-yourself system for dynamic stem cell and organoid culture in standard multi-well plates
Matthias Lütolf, Sebastian Maerkl, Hao-An Hsiung, Zoe Newell Swank, Stefano Davide Vianello, Julia Tischler
We present a low-cost, do-it-yourself system for complexmammalian cell culture under dynamically changing medium formulations by integrating conventional multi-well tissue culture plates with simple microfluidic control and system automation. We demonstrat ...
2022In vitro endoderm emergence and self-organisation in the absence of extraembryonic tissues and embryonic architecture
In humans, mice, and other mammals key internal organs such as the gut, the lungs, the pancreas, and the liver all derive from the same embryonic tissue: the endoderm. The development of all of these structures thus depends on a same set of early cells, an ...
EPFL2022Differential impact of TGF beta/SMAD signaling activity elicited by Activin A and Nodal on endoderm differentiation of epiblast stem cells
Allocation of cells to an endodermal fate in the gastrulating embryo is driven by Nodal signaling and consequent activation of TGF beta pathway. In vitro methodologies striving to recapitulate the process of endoderm differentiation, however, use TGF beta ...
WILEY2022Targeted search for scaling genes reveals matrix metalloproteinase 3 as a scaler of the dorsal-ventral pattern in Xenopus laevis embryos
How embryos scale patterning according to size is still not fully understood. Through in silico screening and analysis of reaction-diffusion systems that could be responsible for scaling, we predicted the existence of genes whose expression is sensitive to ...
2022Deconstructing body axis morphogenesis in zebrafish embryos using robot-assisted tissue micromanipulation
Andrew Charles Oates, Mahmut Selman Sakar, Ece Özelçi, Erik Mailand, Matthias Rüegg
Classic microsurgical techniques, such as those used in the early 1900s by Mangold and Spemann, have been instrumental in advancing our understanding of embryonic development. However, these techniques are highly specialized, leading to issues of inter-ope ...
2022Defined matrices for epithelial organoid culture and disease modelling
Advances in our understanding of the biology of stem cells and their niches have provided the necessary foundation to create in vitro complex self-organizing three-dimensional (3D) structures called organoids. While organoids represent a highly promising n ...
EPFL2021Mechanical control of tissue shape and morphogenetic flows during vertebrate body axis elongation
Shaping embryonic tissues into their functional morphologies requires cells to control the physical state of the tissue in space and time. While regional variations in cellular forces or cell proliferation have been typically assumed to be the main physica ...
2021