Concept

Reverse transcription polymerase chain reaction

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Increasing the protein production capacity of the PURE cell-free transcription-translation (TX-TL) system will be key to implementing complex synthetic biological circuits, and to establishing a fully self-regenerating system as a basis for the development ...

AMER CHEMICAL SOC2022

Derivative voltammetry: a simple tool to probe reaction selectivity in photoelectrochemical cells

Kevin Sivula, Florent Alexandre Boudoire, Nukorn Plainpan

We demonstrate the use of the derivative voltammogram method to predict the selectivity of the photo-driven oxidation of 5-hydroxymethylfurfural (HMF) on a WO3 photoanode as a function of applied potential using only information obtained from the linear sw ...

2022

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Background Organohalide respiration (OHR) is a bacterial anaerobic process in which chlorinated compound, e.g. tetrachloroethene (PCE), is used as terminal electron acceptors. Dehalobacter restrictus PER-K23, a paradigmatic organohalide-respiring bacterium ...

2021

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The production of enantiopure materials and molecules is of uttermost relevance in research and industry in numerous contexts, ranging from nonlinear optics to asymmetric synthesis. In the context of the latter, dehalogenation, which is an essential reacti ...

WILEY-V C H VERLAG GMBH2021

UV disinfection of human norovirus: evaluating infectivity using a genome-wide PCR-based approach

Tamar Kohn, Krista Rule Wigginton, Brian Pecson, Suzanne Marie Young

The removal and inactivation of infectious human norovirus is a major focus in water purification, but its fate through disinfection treatment processes is largely unknown owing to the lack of a readily available infectivity assay. In particular, norovirus ...

2020

MicroRNA-204-5p modulates mitochondrial biogenesis in C2C12 myotubes and associates with oxidative capacity in humans

Xu Wang

Using an unbiased high-throughput microRNA (miRNA)-silencing screen combined with functional readouts for mitochondrial oxidative capacity in C2C12 myocytes, we previously identified 19 miRNAs as putative regulators of skeletal muscle mitochondrial metabol ...

2020

High-throughput microfluidic platforms for characterizing and engineering cell-free gene regulatory circuits

Zoe Newell Swank

Cell-free systems have emerged as a versatile platform for constructing complex biological systems from the bottom-up. In particular, they enable the rapid engineering and characterization of gene regulatory networks, a critical cellular subsystem that all ...

EPFL2020

Bacilysin, as the simplest peptide antibiotic made up of only L-alanine and L-anticapsin, is produced and excreted by Bacillus subtilis under the control of quorum sensing. We analyzed bacilysin-nonproducing strain OGU1 which was obtained by bacA-targeted ...

MICROBIOLOGICAL SOCIETY KOREA2020

Fryns type mesomelic dysplasia of the upper limbs caused by inverted duplications of the HOXD gene cluster

Denis Duboule

The HoxD cluster is critical for vertebrate limb development. Enhancers located in both the telomeric and centromeric gene deserts flanking the cluster regulate the transcription of HoxD genes. In rare patients, duplications, balanced translocations or inv ...

NATURE PUBLISHING GROUP2020

The Metal Catalyst Influences the Kinetics and Mechanisms of MS2 Inactivation in Fenton-like Systems

Tamar Kohn, Jessica Ivana Nieto Juarez

Human enteric viruses are frequent microbial contaminants of surface water and groundwater. Waterborne viruses can be effectively inactivated by oxidants, such as those generated in Fenton-like systems. However, the mechanisms by which this inactivation oc ...

2020