Concept

Reverse transcription polymerase chain reaction

Related publications (178)

Functional genotyping of Sulfurospirillum spp. in mixed cultures allowed the identification of a new tetrachloroethene reductive dehalogenase

Christof Holliger, Julien Maillard, Géraldine Florence Buttet

Background Several enrichment cultures were investigated for their bioremediation potential of tetrachloroethene (PCE). The underlying process (organohalide respiration, OHR) is an anaerobic bacterial respiration during which the chlorinated compounds are ...

2013

Corrinoid auxotrophy in the obligate organohalide respiring Dehalobacter restrictus

Christof Holliger, Julien Maillard, Aamani Rupakula Boyanapalli, Kiyan Shabestary

Background Corrinoids are an essential cofactor of reductive dehalogenases, the key enzymes of the environmental-friendly process of organohalide respiration (OHR). Dehalobacter restrictus strain PER-K23 is an obligate OHR bacterium (OHRB) able to conserve ...

2013

Mechanisms of Human Adenovirus Inactivation by Sunlight and UVC Light as Examined by Quantitative PCR and Quantitative Proteomics

Tamar Kohn, Romain Hamelin, Florence Armand, Franziska Sara Bosshard

Human adenoviruses (HAdV) are important pathogens in both industrialized and developing nations. HAdV has been shown to be relatively resistant to monochromatic UVC. Polychromatic UVC, in contrast, is a more effective means of disinfection, presumably due ...

American Society for Microbiology2013

Development and application of experimental tools to elucidate the gene regulatory network underlying adipogenesis

Carine Delattre-Gubelmann

Excess fat mass in obese individuals, which is characterized by an increase of white adipocyte cell size and number, significantly raises the risk of developing metabolic syndrome symptoms as well as cancer. A detailed understanding of the transcriptional ...

EPFL2013

Identification of HMX1 target genes: A predictive promoter model approach

Purpose: A homozygous mutation in the H6 family homeobox 1 (HMX1) gene is responsible for a new oculoauricular defect leading to eye and auricular developmental abnormalities as well as early retinal degeneration (MIM 612109). However, the HMX1 pathway rem ...

Molecular Vision2013

Abcb4 acts as multixenobiotic transporter and active barrier against chemical uptake in zebrafish (Danio rerio) embryos

Kristin Schirmer

Background: In mammals, ABCB1 constitutes a cellular "first line of defense" against a wide array of chemicals and drugs conferring cellular multidrug or multixenobiotic resistance (MDR/MXR). We tested the hypothesis that an ABCB1 ortholog serves as protec ...

Biomed Central Ltd2013

Changes in the Bacterial Community and lin Genes Diversity during Biostimulation of Indigenous Bacterial Community of Hexachlorocyclohexane (HCH) Dumpsite Soil

Christof Holliger

In this study hexachlorocyclohexane (HCH) contaminated soil (with HCH level 84 g/kg of soil) from HCH dumpsite (Ummari village, Lucknow, India) was used to demonstrate biostimulation approach for HCH bioremediation. Different nutrients (molasses and ammoni ...

Springer Verlag2013

Retinal pigment epithelium protein of 65 kDA gene-linked retinal degeneration is not modulated by chicken acidic leucine-rich epidermal growth factor-like domain containing brain protein/Neuroglycan C/chondroitin sulfate proteoglycan 5

Pascal Escher

Purpose: To analyze in vivo the function of chicken acidic leucine-rich epidermal growth factor-like domain containing brain protein/Neuroglycan C (gene symbol: Cspg5) during retinal degeneration in the Rpe65(-/-) mouse model of Leber congenital amaurosis. ...

Molecular Vision2013

MAR-mediated integration of plasmid vectors for in vivo gene transfer and regulation

Andrzej Kulik, Nicolas Mermod, Stefania Puttini, Déborah Ley, Damien Saugy

Background: The in vivo transfer of naked plasmid DNA into organs such as muscles is commonly used to assess the expression of prophylactic or therapeutic genes in animal disease models. Results: In this study, we devised vectors allowing a tight regulatio ...

BioMed Central2013

Rapid detection of bacterial resistance to antibiotics using AFM cantilevers as nanomechanical sensors

Giovanni Dietler, Sandor Kasas, Giovanni Longo, Livan Bladimir Alonso Sarduy, Laura Rio Marques, Justin Terence Notz Pekkanen

The widespread misuse of drugs has increased the number of multiresistant bacteria1, and this means that tools that can rapidly detect and characterize bacterial response to antibiotics are much needed in the management of infections. Various techniques, s ...

Nature Publishing Group2013

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