Concept
Subculture (biology)
Related publications (14)
Development of 3,5-Dinitrobenzylsulfanyl-1,3,4-oxadiazoles and Thiadiazoles as Selective Antitubercular Agents Active Against Replicating and Nonreplicating Mycobacterium tuberculosis
Stewart Cole, Anthony Vocat, Edina Rita Székely
Herein, we report the discovery and structure activity relationships of 5-substituted-2-[(3,5-dinitrobenzyl)-sulfanyl]-1,3,4-oxadiazoles and 1,3,4-thiadiazoles as a new class of antituberculosis agents. The majority of these compounds exhibited outstanding ...
American Chemical Society2016Echovirus 11 mutants with increased resistance to common water disinfectants
Tamar Kohn, Anna Carratala Ripolles, Virginie Bachmann, Qingxia Zhong
Common water disinfectants like chlorine have been reported to select for resistant viruses. Yet, only little attention has been devoted to characterizing disinfection resistance. The goal of this study was to produce disinfectant-resistant viruses and to ...
2016Echovirus 11 mutants with increased resistance to common water disinfectants
Tamar Kohn, Anna Carratala Ripolles, Virginie Bachmann, Qingxia Zhong
Common water disinfectants like chlorine have been reported to select for resistant viruses. Yet, only little attention has been devoted to characterizing disinfection resistance. The goal of this study was to produce disinfectant-resistant viruses and to ...
2016Recombinant protein production from stable mammalian cell lines and pools
David Hacker, Sowmya Balasubramanian
We highlight recent developments for the production of recombinant proteins from suspension-adapted mammalian cell lines. We discuss the generation of stable cell lines using transposons and lentivirus vectors (non-targeted transgene integration) and site- ...
Elsevier2016Microfluidic chip for monitoring Ca2+ transport through a confluent layer of intestinal cells
Martinus Gijs, Paolo Silacci, Hüseyin Cumhur Tekin, Josias Basil Wacker, Christine Ruffert, Guy Vergères
The absorption of dietary calcium through the intestinal barrier is essential for maintaining health in general and especially of the bone system. We propose a microfluidic model that studies free calcium (Ca2+) transport through a confluent monolayer of C ...
Royal Soc Chemistry2014Actin filament dynamics impacts keratinocyte stem cell maintenance
Cultured human epidermal keratinocyte stem cells (holoclones) are crucial for regenerative medicine for burns and genetic disorders. In serial culture, holoclones progressively lose their proliferative capacity to become transient amplifying cells with lim ...
Wiley Open Access2013PHOTOCATALYTIC DISINFECTION TREATMENTS: VIABILITY, CULTIVABILITY AND METABOLIC CHANGES OF E. coli USING DIFERENT MESUREMENTS METHODS
In this work, the oxidative stress was produced by the illumination of either fixed TiO2 or suspended TiO2. and the relation between cultivability and viability under oxidative conditions was studied using a Direct Viable Count (DVC) and Fluorescence In Si ...
2011Bioengineered 3D platform to explore cell-ECM interactions and drug resistance of epithelial ovarian cancer cells
The behaviour of cells cultured within three-dimensional (3D) structures rather than onto two-dimensional (2D) culture plastic more closely reflects their in vivo responses. Consequently, 3D culture systems are becoming crucial scientific tools in cancer c ...
Elsevier2010Lentiviral Vector-Mediated Gene Delivery for the Generation of Chinese Hamster Ovary Cell Lines with Improved Productivity and Stability
Recombinant cell line generation by standard transfection techniques is a time-consuming and labor-intensive process often leading to an unpredictable outcome as transgene integration is a rare, random event. Consequently, the population of cells obtained ...
EPFL2010A novel method of dynamic culture surface expansion improves mesenchymal stem cell proliferation and phenotype
Hicham Majd, Boris Hinz, Lara Buscemi Estefanell, Pierre Jean Arnould Wipff
Repeated passaging in conventional cell culture reduces pluripotency and proliferation capacity of human mesenchymal stem cells (MSC). We introduce an innovative cell culture method whereby the culture surface is dynamically enlarged during cell proliferat ...
2008