Concept

Primary and secondary antibodies

Related publications (20)

Magnetic beads retention device for sandwich immunoassay: comparison of off-chip and on-chip antibody incubation

Martinus Gijs, Caroline Vandevyver, Frédéric Lacharme

We use magnetic microbeads, which are magnetically self-assembled in chains in a microfluidic chip, as reaction substrates to implement two different sandwich immunoassay protocols for the detection of mouse monoclonal target antibodies. The magnetic chain ...

Springer-Verlag2009

Fixed bed reactor for solid phase surface derivatization of superparamagnetic nanoparticles

Heinrich Hofmann, Jatuporn Salaklang, Alke Fink, Benedikt Steitz, Andrija Finka

The functionalization of nanoparticles is conditio sine qua non in studies of specific interaction with a biological target. Often, their biological functionality is achieved by covalent binding of bioactive molecules on a preexisting single surface coatin ...

2007

Controlled protein adsorption for microimmunoassays

Andrea Lionello

This dissertation addresses the adsorption of proteins in microchannels with the aim of improving the result of an immunoassay. To observe the adsorption of fluorescently labelled immunoglobulins G in laser ablated polymer microchannels, a confocal microsc ...

EPFL2006

The Colored Revolution of Bioimaging

Michaël Unser, Cédric René Jean Vonesch, François Aguet

With the recent development of fluorescent probes and new high-resolution microscopes, biological imaging has entered a new era and is presently having a profound impact on the way research is being conducted in the life sciences. Biologists have come to d ...

IEEE2006

Heterogeneous oligonucleotide-hybridization assay based on hot electron-induced eletrochemiluminescence of a rhodamine label at oxide-coated aluminium and silicon electrodes

Nico de Rooij

This paper describes a heterogeneous oligonucleotide-hybridization assay based on hot electron-induced electrochemiluminescence (HECL) of a rhodamine label. Thin oxide-film coated aluminum and silicon electrodes were modified with an aminosilane layer and ...

2006

Immunohistochemistry for the MEPHISTO X-PEEM

Giorgio Margaritondo

Over almost 50 years of its development, the science of immunology has become an indispensable tool for the understanding of the histology of tissues. Antibodies are highly specific probes, so that tissue structure can be interpreted not just by morphologi ...

Amer Inst Physics2000

Batch cultures of a hybridoma cell line performed with in situ ammonia removal

Urs von Stockar, Ian William Marison, Markus Schneider

Batch cultures of a mouse/mouse hybridoma cell line secreting a dimeric IgA were performed in combination with a membrane process for the continuous removal of ammonia from the culture medium. The latter involves hydrophobic porous membranes with an assocd ...

1997

Photoimmunodiagnosis with antibody-fluorescein conjugates: In vitro and in vivo preclinical studies

Hubert van den Bergh, Georges Wagnières, André Châtelain

The use of dyes has been helpful for the photodiagnosis of small cancers accessible to endoscopic examn. An important limiting factor of this technol. is that the presently used fluorescent dye mixt. has a relatively poor capacity to accumulate preferentia ...

1992

Antibody-fluorescein conjugates for photoimmunodiagnosis of human colon carcinoma in nude mice

Hubert van den Bergh, Georges Wagnières

To improve the detectability of tumors by light-induced fluorescence, the use of monoclonal antibodies (MoAb) as carriers of fluorescent molecules was studied. As a model for this approach, the biodistribution of an anticarcinoembryonic antigen (CEA) MoAb ...

1991

Specific pancreatic beta-cell surface antigens recognized by a xenogenic antiserum

Steinunn Baekkeskov Hanahan

An antiserum (R4) from a rabbit immunized with suspensions of C57BL/61 ob/ob mouse islet cells contains antibodies which in a 125I-protein A radioligand assay can be demonstrated to bind to single cell suspensions of normal Naval Medical Research Institute ...

1982

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